Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee , Dundee, UK.
Cell Cycle. 2020 Jun;19(12):1422-1432. doi: 10.1080/15384101.2020.1757242. Epub 2020 May 13.
Until recently, our understanding of chromosome organization in higher eukaryotic cells has been based on analyses of large-scale, low-resolution changes in chromosomes structure. More recently, CRISPR-Cas9 technologies have allowed us to "zoom in" and visualize specific chromosome regions in live cells so that we can begin to examine in detail the dynamics of chromosome organization in individual cells. In this review, we discuss traditional methods of chromosome locus visualization and look at how CRISPR-Cas9 gene-targeting methodologies have helped improve their application. We also describe recent developments of the CRISPR-Cas9 technology that enable visualization of specific chromosome regions without the requirement for complex genetic manipulation.
直到最近，我们对高等真核细胞中染色体组织的理解还一直基于对染色体结构的大规模、低分辨率变化的分析。最近，CRISPR-Cas9 技术使我们能够“放大”并在活细胞中可视化特定的染色体区域，从而使我们能够开始详细研究单个细胞中染色体组织的动态。在这篇综述中，我们讨论了传统的染色体基因座可视化方法，并探讨了 CRISPR-Cas9 基因靶向方法学如何帮助改进它们的应用。我们还描述了 CRISPR-Cas9 技术的最新进展，这些进展使得无需复杂的遗传操作即可实现特定染色体区域的可视化。
