Ellen Albert F, Albers Sonja-Verena, Huibers Wim, Pitcher Angela, Hobel Cedric F V, Schwarz Heinz, Folea Mihaela, Schouten Stefan, Boekema Egbert J, Poolman Bert, Driessen Arnold J M
Department of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Extremophiles. 2009 Jan;13(1):67-79. doi: 10.1007/s00792-008-0199-x. Epub 2008 Oct 31.
The crenarchaea Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii, release membrane vesicles into the medium. These membrane vesicles consist of tetraether lipids and are coated with an S-layer. A proteomic analysis reveals the presence of proteins homologous to subunits of the eukaryotic endosomal sorting complex required for transport (ESCRT). Immunodetection of one of these homologs suggest a cell surface localization in intact cells. These data suggest that the membrane vesicles in Sulfolobus sp. emerge from a specific budding process with similarity to the endosomal sorting pathway.
嗜热栖热菌、嗜酸热硫化叶菌和托科达伊硫化叶菌这几种泉古菌会向培养基中释放膜泡。这些膜泡由四醚脂质构成，并被一层S层覆盖。蛋白质组学分析显示存在与真核生物运输所需内体分选复合体（ESCRT）亚基同源的蛋白质。对其中一种同源物的免疫检测表明其在完整细胞中定位于细胞表面。这些数据表明，硫化叶菌属中的膜泡源自一个与内体分选途径相似的特定出芽过程。
