Molecular Biology of Archaea, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany.
J Proteome Res. 2011 Sep 2;10(9):4105-19. doi: 10.1021/pr2003006. Epub 2011 Aug 1.
Microorganisms in nature often live in surface-associated sessile communities, encased in a self-produced matrix, referred to as biofilms. Biofilms have been well studied in bacteria but in a limited way for archaea. We have recently characterized biofilm formation in three closely related hyperthermophilic crenarchaeotes: Sulfolobus acidocaldarius, S. solfataricus, and S. tokodaii. These strains form different communities ranging from simple carpet structures in S. solfataricus to high density tower-like structures in S. acidocaldarius under static condition. Here, we combine spectroscopic, proteomic, and transcriptomic analyses to describe physiological and regulatory features associated with biofilms. Spectroscopic analysis reveals that in comparison to planktonic life-style, biofilm life-style has distinctive influence on the physiology of each Sulfolobus spp. Proteomic and transcriptomic data show that biofilm-forming life-style is strain specific (eg ca. 15% of the S. acidocaldarius genes were differently expressed, S. solfataricus and S. tokodaii had ~3.4 and ~1%, respectively). The -omic data showed that regulated ORFs were widely distributed in basic cellular functions, including surface modifications. Several regulated genes are common to biofilm-forming cells in all three species. One of the most striking common response genes include putative Lrs14-like transcriptional regulators, indicating their possible roles as a key regulatory factor in biofilm development.
自然界中的微生物通常生活在表面相关的固着群落中，被包裹在自身产生的基质中，这种基质被称为生物膜。生物膜在细菌中已经得到了很好的研究，但在古菌中研究得很少。我们最近对三种密切相关的高温嗜热古菌：嗜酸热硫化叶菌、热泉硫化叶菌和塔氏高温古菌的生物膜形成进行了表征。在静态条件下，这些菌株形成不同的群落，从热泉硫化叶菌中的简单地毯结构到嗜酸热硫化叶菌中的高密度塔式结构。在这里，我们结合光谱学、蛋白质组学和转录组学分析来描述与生物膜相关的生理和调节特征。光谱分析表明，与浮游生活方式相比，生物膜生活方式对每个 Sulfolobus spp 的生理有独特的影响。蛋白质组学和转录组学数据表明，生物膜形成的生活方式是菌株特异性的（例如，约 15%的嗜酸热硫化叶菌基因表达不同，热泉硫化叶菌和塔氏高温古菌分别有3.4%和1%）。这些组学数据表明，受调控的 ORF 广泛分布于基本的细胞功能中，包括表面修饰。几个受调控的基因在所有三种生物膜形成细胞中都是共同的。其中最引人注目的共同响应基因之一包括假定的 Lrs14 样转录调节因子，表明它们可能在生物膜发育中作为关键调节因子发挥作用。
