Segawa Yuko, Muneta Takeshi, Makino Hatsune, Nimura Akimoto, Mochizuki Tomoyuki, Ju Young-Jin, Ezura Yoichi, Umezawa Akihiro, Sekiya Ichiro
Section of Orthopedic Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
J Orthop Res. 2009 Apr;27(4):435-41. doi: 10.1002/jor.20786.
Mesenchymal stem cells (MSCs) can be obtained from various tissues, and contain common features. However, an increasing number of reports have described variant properties dependent of cell sources. We examined (1) whether MSCs existed in several intraarticular tissues, (2) whether gene expression profiles in intraarticular tissue MSCs closely resembled each other, and (3) whether identified genes were specific to intraarticular tissue MSCs. Human synovium, meniscus, intraarticular ligament, muscle, adipose tissue, and bone marrow were harvested, and colony-forming cells were analyzed. All these cells showed multipotentiality and surface markers typical of MSCs. Gene profiles of intraarticular tissue MSCs and chondrocytes were closer to each other than those of extraarticular tissues MSCs. Among three characteristic genes specific for intraarticular tissue MSCs, we focused on proline arginine-rich end leucine-rich repeat protein (PRELP). Higher expression of PRELP was confirmed in chondrocytes and intraarticular tissue MSCs among three elderly and three young donors. Synovium MSCs stably expressed PRELP, contrarily, bone marrow MSCs increased PRELP expression during in vitro chondrogenesis. In conclusion, MSCs could be isolated from various intraarticular tissues including meniscus and ligament, gene expression profiles of intraarticular tissue MSCs closely resembled each other, and the higher expression of PRELP was characteristic of intraarticular tissue MSCs.
间充质干细胞（MSCs）可从多种组织中获取，并具有共同特征。然而，越来越多的报告描述了依赖于细胞来源的变异特性。我们研究了：（1）关节内多种组织中是否存在MSCs；（2）关节内组织MSCs的基因表达谱是否彼此高度相似；（3）所鉴定的基因是否为关节内组织MSCs所特有。采集了人类滑膜、半月板、关节内韧带、肌肉、脂肪组织和骨髓，并对集落形成细胞进行了分析。所有这些细胞均表现出MSCs典型的多能性和表面标志物。关节内组织MSCs和软骨细胞的基因谱比关节外组织MSCs的基因谱彼此更接近。在关节内组织MSCs特有的三个特征基因中，我们重点关注富含脯氨酸精氨酸的富含亮氨酸重复蛋白（PRELP）。在三名老年供体和三名年轻供体中，软骨细胞和关节内组织MSCs中PRELP的表达均较高得到证实。滑膜MSCs稳定表达PRELP，相反，骨髓MSCs在体外软骨形成过程中PRELP表达增加。总之，MSCs可从包括半月板和韧带在内的多种关节内组织中分离出来，关节内组织MSCs的基因表达谱彼此高度相似，且PRELP的高表达是关节内组织MSCs的特征。
