多聚甲醛固定对软骨延迟钆增强磁共振成像（dGEMRIC）测量的影响。
The effect of paraformaldehyde fixation on the delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) measurement.
作者信息
Dugar Arushi, Farley Michelle L, Wang Angeline L, Goldring Mary B, Goldring Steven R, Swaim Bryan H, Bierbaum Benjamin E, Burstein Deborah, Gray Martha L
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
出版信息
J Orthop Res. 2009 Apr;27(4):536-9. doi: 10.1002/jor.20767.
The delayed Gadolinium-Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC) method allows for both qualitative and quantitative measurement of the spatial distribution of glycosaminoglycan [GAG] in excised cartilage. The objective of this study was to determine the effect of paraformaldehyde fixation on dGEMRIC measurements. Five bovine and seven human cartilage pieces were punched into 5-mm plugs, fixed for 18 h in 4% paraformaldehyde solution, and washed. The magnetic resonance imaging (MRI) parameter T1 was measured prior and post fixation in cartilage without (T1(0)) and with (T1(Gd)), the ionically charged MRI contrast agent Gd(DTPA)(2-). Images of tissue before and after fixation were qualitatively very similar. The ratios of T1(0), T1(Gd), and calculated [GAG] after fixation, relative to before fixation, were near or slightly higher than 1 for both bovine cartilage (1.01 +/- 0.01, 1.04 +/- 0.02, 1.05 +/- 0.03, respectively) and for human cartilage (0.96 +/- 0.11, 1.03 +/- 0.05, 1.09 +/- 0.13). Thus, these data suggest that dGEMRIC can be used on previously fixed samples to assess the three dimensional spatial distribution of GAG.
延迟钆增强磁共振成像软骨（dGEMRIC）方法可对切除软骨中糖胺聚糖[GAG]的空间分布进行定性和定量测量。本研究的目的是确定多聚甲醛固定对dGEMRIC测量的影响。将五块牛软骨和七块人软骨切成5毫米的小块，在4%多聚甲醛溶液中固定18小时，然后冲洗。在未使用（T1(0)）和使用（T1(Gd)）离子型磁共振成像造影剂钆喷酸葡胺（Gd(DTPA)(2-)）的情况下，分别在固定前后测量软骨的磁共振成像（MRI）参数T1。固定前后组织的图像在质量上非常相似。牛软骨（分别为1.01±0.01、1.04±0.02、1.05±0.03）和人软骨（0.96±0.11、1.03±0.05、1.09±0.13）固定后T1(0)、T1(Gd)以及计算得出的[GAG]与固定前的比值均接近或略高于1。因此，这些数据表明dGEMRIC可用于先前固定的样本，以评估GAG的三维空间分布。
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