de Heens G L Torres, Kikkert R, Aarden L A, van der Velden U, Loos B G
Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), The Netherlands.
J Periodontal Res. 2009 Feb;44(1):28-34. doi: 10.1111/j.1600-0765.2007.01047.x. Epub 2008 Oct 29.
Smoking is associated with increased severity of periodontitis. The underlying mechanisms of this phenomenon are not well understood. The purpose of the present study was to compare the monocyte-derived T cell directing (Th1/Th2) response and pro-inflammatory cytokine production in ex vivo whole blood cell cultures (WBCC) of smoking and non-smoking chronic periodontitis patients.
Venous blood was collected from 29 periodontitis patients (18 non-smokers and 11 smokers) receiving supportive periodontal treatment, and diluted 10-fold for WBCC. The WBCC were stimulated for 18 h with Neisseria meningitidis lipo-oligosaccharide (LOS) or Porphyromonas gingivalis sonic extract (Pg-SE). The production of the T cell directing cytokines interleukin (IL)-12 p40 and IL-10, as well as the pro-inflammatory cytokines IL-1beta, IL-6 and IL-8, was measured in the culture supernatants.
After LOS stimulation of WBCC, smokers showed a lower IL-12 p40/IL-10 ratio than non-smokers (P < 0.05). Interleukin-1beta production was significantly lower in smokers compared with non-smokers after stimulation with either LOS or Pg-SE (P < 0.05). Interleukin-6 and IL-8 production was similar in WBCC from both smokers and non-smokers, for both LOS and Pg-SE.
A more pronounced Th2 response in smoking periodontitis patients may be related to increased severity of the disease.
吸烟与牙周炎严重程度增加有关。这一现象的潜在机制尚未完全明确。本研究的目的是比较吸烟和不吸烟的慢性牙周炎患者体外全血细胞培养（WBCC）中单核细胞衍生的T细胞导向（Th1/Th2）反应及促炎细胞因子的产生。
从29例接受牙周支持治疗的牙周炎患者（18例不吸烟者和11例吸烟者）采集静脉血，并稀释10倍用于WBCC。用脑膜炎奈瑟菌脂寡糖（LOS）或牙龈卟啉单胞菌超声提取物（Pg-SE）刺激WBCC 18小时。检测培养上清液中T细胞导向细胞因子白细胞介素（IL）-12 p40和IL-10以及促炎细胞因子IL-1β、IL-6和IL-8的产生。
LOS刺激WBCC后，吸烟者的IL-12 p40/IL-10比值低于不吸烟者（P<0.05）。LOS或Pg-SE刺激后，吸烟者的IL-1β产生明显低于不吸烟者（P<0.05）。对于LOS和Pg-SE，吸烟者和不吸烟者的WBCC中IL-6和IL-8的产生相似。
吸烟的牙周炎患者中更明显的Th2反应可能与疾病严重程度增加有关。
