吸烟对慢性牙周炎患者牙龈组织中炎症细胞浸润亚型的影响。
The effect of smoking on inflammatory cell infiltrate subtypes in gingival tissue of patients with chronic periodontitis.
作者信息
Radvar Mehrdad, Shafaee Hooman, Mohtasham Nooshin, Shiezadeh Farid, Zamanpour Mona
机构信息
Associate Professor, Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Assistant Professor, Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Electron Physician. 2017 Aug 25;9(8):4961-4967. doi: 10.19082/4961. eCollection 2017 Aug.
BACKGROUND
Examinations on blood samples and gingival crevicular fluid of subjects with chronic periodontitis showed that smoking increased production of cytokines.
OBJECTIVE
To evaluate the expression of immune markers on mononuclear inflammatory cell infiltrate at periodontitis and healthy tissue among smoking and non-smoking subjects.
METHODS
This case-control study was performed on 41 patients who referred to a clinic of periodontology at a Mashhad dental school in Iran in 2016. The participants were all of Iranian Khorasanian ethnicity with age range of 35-65 years. Gingival biopsies were obtained during routine periodontal flap procedure. Immunohistochemistry using markers of CD20, CD3, CD68, and CD45RO was carried out. Data was analyzed by SPSS version 15, using one-way ANOVA and Tukey HSD test.
RESULTS
Nonsmoker subjects showed significantly greater numbers of CD20+, CD68+, CD3+ cells compared to smoker subjects, both at healthy and periodontitis tissue biopsies (p<0.00), whereas there was no significant difference in terms of CD45RO (p=0.120).
CONCLUSION
Cigarette smoking, results in infiltrative mononuclear chronic inflammatory cells reduction in connective periodontium.
背景
对慢性牙周炎患者的血液样本和龈沟液进行检测发现，吸烟会增加细胞因子的产生。
目的
评估吸烟和不吸烟受试者牙周炎组织及健康组织中单核炎性细胞浸润上免疫标志物的表达情况。
方法
本病例对照研究于2016年对转诊至伊朗马什哈德一所牙科学院牙周病诊所的41例患者开展。参与者均为伊朗霍拉桑族，年龄在35 - 65岁之间。在常规牙周翻瓣手术过程中获取牙龈活检组织。采用CD20、CD3、CD68和CD45RO标志物进行免疫组织化学检测。使用SPSS 15.0版软件进行数据分析，采用单因素方差分析和Tukey HSD检验。
结果
在健康组织和牙周炎组织活检中，不吸烟受试者的CD20 +、CD68 +、CD3 +细胞数量均显著多于吸烟受试者（p < 0.00），而CD45RO方面无显著差异（p = 0.120）。
结论
吸烟会导致牙周结缔组织中浸润性单核慢性炎症细胞减少。
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