通过喷雾热解合成的磁性/发光核/壳颗粒及其在带有内标的免疫分析中的应用。

Magnetic/luminescent core/shell particles synthesized by spray pyrolysis and their application in immunoassays with internal standard.

作者信息

Dosev Dosi, Nichkova Mikaela, Dumas Randy K, Gee Shirley J, Hammock Bruce D, Liu Kai, Kennedy Ian M

机构信息

Department of Mechanical and Aeronautical Engineering, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.

出版信息

Nanotechnology. 2007 Jul;18(5):55102. doi: 10.1088/0957-4484/18/5/055102.

Many types of fluorescent nanoparticles have been investigated as alternatives to conventional organic dyes in biochemistry; magnetic beads also have a long history of biological applications. In this work we apply flame spray pyrolysis in order to engineer a novel type of nanoparticle that has both luminescent and magnetic properties. The particles have magnetic cores of iron oxide doped with cobalt and neodymium and luminescent shells of europium-doped gadolinium oxide (Eu:Gd(2)O(3)). Measurements by vibrating sample magnetometry showed an overall paramagnetic response of these composite particles. Luminescence spectroscopy showed spectra typical of the Eu ion in a Gd(2)O(3) host-a narrow emission peak centred near 615 nm. Our synthesis method offers a low-cost, high-rate synthesis route that enables a wide range of biological applications of magnetic/luminescent core/shell particles. Using these particles we demonstrate a novel immunoassay format with internal luminescent calibration for more precise measurements.

在生物化学领域，人们已对多种荧光纳米颗粒进行了研究，将其作为传统有机染料的替代品；磁珠在生物应用方面也有着悠久的历史。在这项工作中，我们应用火焰喷雾热解技术来设计一种新型的纳米颗粒，该颗粒同时具有发光和磁性特性。这些颗粒具有掺杂了钴和钕的氧化铁磁芯以及掺杂了铕的氧化钆（Eu:Gd₂O₃）发光外壳。通过振动样品磁强计测量表明，这些复合颗粒呈现出整体顺磁响应。发光光谱显示出在Gd₂O₃基质中Eu离子典型的光谱——一个以615nm附近为中心的窄发射峰。我们的合成方法提供了一种低成本、高速率的合成途径，能够实现磁性/发光核/壳颗粒在广泛生物应用中的应用。使用这些颗粒，我们展示了一种具有内部发光校准功能的新型免疫分析形式，用于更精确的测量。