Lee Dong Wan, Lee Soon Dong
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
J Microbiol. 2008 Oct;46(5):508-12. doi: 10.1007/s12275-008-0096-0. Epub 2008 Oct 31.
A novel actinobacterium, designated strain MSW-19(T), was isolated from a seawater sample in Republic of Korea. Cells were aerobic, Gram-positive, non-endospore-forming, and non-motile cocci. Colonies were circular, convex, opaque, and vivid yellow in colour. A phylogenetic tree based on 16S rRNA gene sequences exhibited that the organism formed a distinct clade within the radius encompassing representatives of the family Propionibacteriaceae. The phylogenetic neighbors were the type strains of the genera Friedmanniella, Microlunatus, Micropruina, Propionicicella, and Propionicimonas. Levels of 16S rRNA gene sequence similarity between the isolate and members of the family were less than 95.3%. The cell wall peptidoglycan of the organism contained LL-diaminopimelic acid as the diagnostic diamino acid. The isolate contained MK-9(H(4)) as the predominant menaquinone, ai-C(15:0) as the major fatty acid and polar lipids including phosphatidylglycerol, phosphatidylethanolamine, and an unknown phospholipid. The G+C content of the DNA was 69.6 mol%. On the basis of the phenotypic and phylogenetic data presented here, the isolate is considered to represent a novel genus and species in the family Propionibacteriaceae, for which the name Ponticoccus gilvus gen. nov., sp. nov. is proposed. The type strain is strain MSW-19(T) (= KCTC 19476(T)= DSM 21351(T)).
从韩国的一份海水样本中分离出一株新型放线菌，命名为MSW-19(T)菌株。细胞为需氧型、革兰氏阳性、不形成芽孢且无运动性的球菌。菌落呈圆形、凸起、不透明，颜色为鲜黄色。基于16S rRNA基因序列构建的系统发育树显示，该菌株在包含丙酸杆菌科代表菌株的半径范围内形成了一个独特的分支。其系统发育近缘种为弗里德曼氏菌属、微小月形菌属、微丙酸菌属、丙酸栖菌属和丙酸单胞菌属的模式菌株。该分离株与该科成员的16S rRNA基因序列相似性水平低于95.3%。该菌株的细胞壁肽聚糖含有LL-二氨基庚二酸作为诊断性二氨基酸。该分离株以MK-9(H(4))作为主要甲基萘醌，ai-C(15:0)作为主要脂肪酸，以及包括磷脂酰甘油、磷脂酰乙醇胺和一种未知磷脂的极性脂质。DNA的G+C含量为69.6 mol%。基于此处呈现的表型和系统发育数据，该分离株被认为代表了丙酸杆菌科中的一个新属和新种，为此提出新属名为Ponticoccus，新种名为gilvus。模式菌株为MSW-19(T)菌株(= KCTC 19476(T)= DSM 21351(T))。
