Finster K W, Cockell C S, Voytek M A, Gronstal A L, Kjeldsen K U
Department of Biological Sciences, Section for Microbiology, Aarhus University, Building 1540, Ny Munkegade 114, 8000, Aarhus C., Denmark.
Antonie Van Leeuwenhoek. 2009 Nov;96(4):515-26. doi: 10.1007/s10482-009-9367-y. Epub 2009 Aug 8.
A novel actinobacterium, designated CB31(T), was isolated from a 940 m depth sample of a drilling core obtained from the Chesapeake meteor impact crater. The strain was isolated aerobically on R2A medium agar plates supplemented with NaCl (20 g l(-1)) and MgCl2 x 6 H2O (3 g l(-1)). The colonies were circular, convex, smooth and orange. Cells were slightly curved, rod-shaped in young cultures and often appeared in pairs. In older cultures cells were coccoid. Cells stained Gram-positive, were non-motile and did not form endospores. The diagnostic diamino acid of the peptidoglycan was LL: -diaminopimelic acid. The polar lipids included phosphatidylglycerol, diphosphatidglycerol, four different glycolipids, two further phospholipids and one unidentified lipid. The dominant menaquinone was MK-9(H(4)) (70%). The major cellular fatty acid was anteiso C15:0 (83%). The DNA G + C content was 68 mol%. The strain grew anaerobically by reducing nitrate to nitrite or by fermenting glucose. It was catalase positive and oxidase negative. It grew between 10 and 45 degrees C, with an optimum between 35 and 40 degrees C. The pH range for growth was 5.7-9.3, with an optimum at pH 7.5. The closest phylogenetic neighbors based on 16S rRNA gene sequence identity were members of the genus Tessaracoccus (95-96% identity). On the basis of phenotypic and phylogenetic distinctiveness, strain CB31(T) is considered to represent a novel species of the genus Tessaracoccus, for which we propose the name Tessaracoccus profundi sp. nov.. It is the first member of this genus that has been isolated from a deep subsurface environment. The type strain is CB31(T) (=NCIMB 14440(T) = DSM 21240(T)).
从切萨皮克陨石撞击坑获取的深度为940米的钻孔岩芯样本中分离出一株新型放线菌，命名为CB31(T)。该菌株在添加了NaCl（20 g l(-1)）和MgCl2·6H2O（3 g l(-1)）的R2A培养基琼脂平板上需氧培养。菌落呈圆形、凸起、光滑且为橙色。细胞稍弯曲，在年轻培养物中呈杆状，常成对出现。在较老的培养物中细胞呈球状。细胞革兰氏染色阳性，无运动性，不形成芽孢。肽聚糖的诊断性二氨基酸为LL-二氨基庚二酸。极性脂质包括磷脂酰甘油、双磷脂酰甘油、四种不同的糖脂、另外两种磷脂和一种未鉴定的脂质。主要的甲基萘醌是MK-9(H(4))（70%）。主要的细胞脂肪酸是anteiso C15:0（83%）。DNA G + C含量为68 mol%。该菌株通过将硝酸盐还原为亚硝酸盐或发酵葡萄糖进行厌氧生长。它过氧化氢酶阳性，氧化酶阴性。生长温度范围为10至45℃，最适温度为35至40℃。生长的pH范围为5.7 - 9.3，最适pH为7.5。基于16S rRNA基因序列同一性，最接近的系统发育邻居是四联球菌属的成员（同一性为95 - 96%）。基于表型和系统发育的独特性，菌株CB31(T)被认为代表四联球菌属的一个新物种，我们将其命名为深海底四联球菌（Tessaracoccus profundi sp. nov.）。它是该属中首个从深部地下环境中分离出的成员。模式菌株为CB31(T)（=NCIMB 14440(T) = DSM 21240(T)）。
