Kostilenko Yu P, Boiko I V, Starchenko I I, Prilutskii A K
Department of Human Anatomy, Ukraine Medical Stomatological Academy, Poltava, Ukraine.
Neurosci Behav Physiol. 2008 Nov;38(9):897-9. doi: 10.1007/s11055-008-9067-5. Epub 2008 Oct 31.
Effective methods for making semithin sections of large surface area are not currently available. An alternative solution to this problem, without actual histological section preparation, is proposed. This is a modification of a combination of methods for fixing tissues and embedding them in epoxide resin using known technical methods. This method for making preparations for microscopic examination of biological objects with large surface areas has additional valuable potential, as it allows investigations of a variety of complexes of soft and hard (cartilaginous and bony) tissues without prior decalcination.
目前还没有制作大表面积半薄切片的有效方法。本文提出了一种无需实际组织学切片制备的替代解决方案。这是对使用已知技术方法固定组织并将其包埋在环氧树脂中的方法组合的改进。这种用于制备大表面积生物对象显微镜检查标本的方法具有额外的重要潜力，因为它允许在不预先脱钙的情况下研究各种软、硬（软骨和骨）组织复合体。
