Botti F, Martignoni M, Scala C, Cocchia D
Cattedra di Istituzioni di Anatomia Umana Normale e dell'Apparato Stomatognatico, Università degli Studi di Roma Tor Vergata, Roma.
Minerva Stomatol. 1995 Apr;44(4):145-53.
Resin embedding of human teeth for light and transmission electron microscopic studies becomes difficult without previous decalcification. The limited and slow infiltration of the resin into hard tissues may cause problems during preparation and observation of the samples. Moreover the type of resin that is used may affect the morphologic preservation of both tissues and cellular elements. Recently there has been an increasing number of studies on the application of acrylic resins in light and electron microscopy, in order to overcome problems encountered with the use of epoxy resins still utilized in morphologic studies. We compared different acrylic resins (Technovit 7200 VLC, LR White, LR Gold, Bioacryl) in order to understand which one was more suitable for undecalcified human dental tissues under light and transmission electron microscope. Evaluation of such resins was performed using the following criteria: ease of cutting with ultramicrotome, soft and hard tissues infiltration, uptake of tissue stains for both light and electron microscopy, morphologic preservation and stability under electron beam. This study, carried out on the pulp area comprising predentin and dentin, showed excellent quality of Bioacryl and LR Gold, the two resins presenting, by far, the best results among all the different types tested. The optimal morphologic preservation obtained with such resins is indicated for light and electron microscopic studies, allowing their application in different fields of dental research.
对于光镜和透射电镜研究而言，未经预先脱钙的人类牙齿进行树脂包埋变得困难。树脂向硬组织的有限且缓慢渗透可能在样本制备和观察过程中引发问题。此外，所使用的树脂类型可能会影响组织和细胞成分的形态保存。近来，为了克服形态学研究中仍在使用的环氧树脂所遇到的问题，关于丙烯酸树脂在光镜和电镜中的应用研究日益增多。我们比较了不同的丙烯酸树脂（Technovit 7200 VLC、LR White、LR Gold、Bioacryl），以了解哪一种在光镜和透射电镜下更适合未脱钙的人类牙齿组织。使用以下标准对这些树脂进行评估：用超薄切片机切割的难易程度、软硬组织的渗透情况、光镜和电镜下组织染色的摄取情况、形态保存以及在电子束下的稳定性。这项针对包含前期牙本质和牙本质的牙髓区域进行的研究表明，Bioacryl和LR Gold质量优异，在所有测试的不同类型树脂中，这两种树脂目前呈现出最佳结果。用这些树脂获得的最佳形态保存适用于光镜和电镜研究，使其能够应用于牙科研究的不同领域。
