Lee S J, Kim H P, Park B K, Ahn S C, Lee H S, Ahn J S
College of Pharmacy, Kangwon National University, 200-701, Chuncheon, Korea.
Arch Pharm Res. 1997 Aug;20(4):372-4. doi: 10.1007/BF02976203.
In order to develop new anti-inflammatory agents having different action mechanisms compared with nonsteroidal and steroidal anti-inflammatory drugs, the culture broths of various actinomycetes isolated from soil were screened using anin vivo mouse ear edma assay and one strain (Streptomyces sp. MT 2705-4: KCTC 8651P) was selected. Activity-guided purification led to the isolation of a polyether compound, dianemycin. Topically, dianemycin showed a potent anti-inflammatory activity in mouse ear edema induced by croton-oil or arachidonic acid. ED(50) value of dianemycin was found to be 0.8 mg/ear compared to 0.4 mg/ear of prednisolone in croton-oil ear edema. However, dianemycin did not show the inhibitory activity in UV-erythema and delayed hypersensitivity reaction. These results indicate that dianemycin is a potential topical anti-inflammatory agent.
为了开发与非甾体和甾体抗炎药作用机制不同的新型抗炎药，使用体内小鼠耳水肿试验对从土壤中分离出的各种放线菌的培养液进行筛选，选出了一株（链霉菌属MT 2705-4：KCTC 8651P）。通过活性导向纯化分离出一种聚醚化合物——地恩霉素。局部应用时，地恩霉素在巴豆油或花生四烯酸诱导的小鼠耳水肿中显示出强大的抗炎活性。在巴豆油耳水肿实验中，地恩霉素的ED（50）值为0.8mg/耳，而泼尼松龙为0.4mg/耳。然而，地恩霉素在紫外线红斑和迟发型超敏反应中未显示出抑制活性。这些结果表明地恩霉素是一种潜在的局部抗炎药。
