Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, India.
Environ Technol. 2011 Jan;32(1-2):83-91. doi: 10.1080/09593330.2010.487547.
Bitter gourd peroxidase entrapped on calcium-alginate-starch beads was employed for the treatment of water, polluted with benzidine, in a batch process as well as in a continuous reactor. The immobilized enzyme had the same pH and temperature optima as the soluble enzyme. The immobilized enzyme could effectively remove more than 70% of benzidine in a stirred batch process after three hours. After its sixth repeated use, the immobilized enzyme was able to oxidatively degrade and polymerize 58% of benzidine. The horizontal bed reactor, containing the peroxidase entrapped on calcium-alginate-starch beads, retained more than 40% benzidine removal efficiency after one month of its continuous operation. The absorption spectra of the treated benzidine exhibited a marked difference in the absorption at various wavelengths, compared with untreated compound. The horizontal bed reactor containing immobilized peroxidase would be significantly successful for the large-scale treatment of water polluted with aromatic compounds.
苦瓜过氧化物酶固定在海藻酸钠-淀粉珠上，用于处理含联苯胺的水，在分批处理和连续反应器中均有应用。固定化酶的 pH 值和最适温度与可溶酶相同。在搅拌分批处理三小时后，固定化酶能有效去除超过 70%的联苯胺。重复使用六次后，固定化酶仍能氧化降解和聚合 58%的联苯胺。含固定化过氧化物酶的水平床反应器在连续运行一个月后，仍能保持超过 40%的联苯胺去除效率。与未处理的化合物相比，处理后的联苯胺的吸收光谱在各个波长的吸收有明显差异。含固定化过氧化物酶的水平床反应器在大规模处理芳香族化合物污染的水方面将非常成功。
