Mathur S, Chao L, Schulte B A, Woodley-Miller C, Abdel-Latif A, Fredericks C M, Rust P F, Williamson H O
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston 29425.
Arch Androl. 1987;19(2):161-75. doi: 10.3109/01485018708986812.
Adult male rabbits were immunized with normal saline (controls), sperm extracts from 2 autoimmune men, seminal plasmas from the same autoimmune men, sperm extract from a fertile nonautoimmune man, and seminal plasma from the same fertile nonautoimmune man. All the sperm donors were free from infections. Rabbits immunized with fertile men's sperm extract and seminal plasma had significantly elevated postimmunization hemagglutinating but not cytotoxic sperm antibodies and reproduced normally. Rabbits immunized with autoimmune men's sperm and seminal plasma antigens developed high titers of cytotoxic and hemagglutinating sperm antibodies in their serum and seminal plasma and their reproduction was markedly reduced. Their sera and seminal plasma reduced motility of sperm from a normal donor. The immune responses were confirmed by electron microscopic immunocytochemistry. This technique revealed membrane-bound endogenous IgG on sperm from only those rabbits immunized with sperm extracts from autoimmune men. These antisera reacted against a protein in the 58,000 D range; antisera to fertile man's sperm extract reacted against three proteins with molecular weights of 15,000, 18,400, 25,000, and 44,000 D, as judged by Western blot. Rabbit antisera to seminal plasma from autoimmune men reacted against several proteins; additionally, it detected two proteins with 43,000 and 68,000 molecular weight detected by antiserum to fertile man's seminal plasma. Sperm and seminal plasma antigens from autoimmune men are different in their elicited immunogenic responses from those of fertile nonautoimmune men. These responses are relevant to infertility.
成年雄性兔子分别用生理盐水（对照组）、两名自身免疫性男性的精子提取物、同一两名自身免疫性男性的精浆、一名可育非自身免疫性男性的精子提取物以及同一可育非自身免疫性男性的精浆进行免疫。所有精子捐献者均无感染。用可育男性的精子提取物和精浆免疫的兔子在免疫后血凝性精子抗体显著升高，但细胞毒性精子抗体未升高，且繁殖正常。用自身免疫性男性的精子和精浆抗原免疫的兔子血清和精浆中产生了高滴度的细胞毒性和血凝性精子抗体，其繁殖能力明显降低。它们的血清和精浆降低了正常供体精子的活力。免疫反应通过电子显微镜免疫细胞化学得到证实。该技术仅在那些用自身免疫性男性的精子提取物免疫的兔子的精子上发现了膜结合内源性IgG。这些抗血清与58,000 D范围内的一种蛋白质发生反应；根据蛋白质印迹法判断，针对可育男性精子提取物的抗血清与分子量为15,000、18,400、25,000和44,000 D的三种蛋白质发生反应。针对自身免疫性男性精浆的兔抗血清与几种蛋白质发生反应；此外，它还检测到了针对可育男性精浆抗血清所检测到的分子量为43,000和68,000的两种蛋白质。自身免疫性男性的精子和精浆抗原引发的免疫反应与可育非自身免疫性男性的不同。这些反应与不育症相关。
