Peeters E, Nelis H J, Coenye T
Laboratory for Pharmaceutical Microbiology, Ghent University, Ghent, Belgium.
J Hosp Infect. 2008 Dec;70(4):361-8. doi: 10.1016/j.jhin.2008.08.015. Epub 2008 Nov 1.
In the present study we evaluated the efficacy of various procedures recommended for the disinfection of respiratory equipment and other materials in cystic fibrosis, using both planktonic and sessile Burkholderia cenocepacia cells. A modified European Suspension Test was performed to determine the effects of the disinfection procedures on planktonic cells. The ability of the treatments to kill sessile cells and to remove biofilm biomass was evaluated using two resazurin-based viability assays and a crystal violet staining on biofilms grown and treated in 96-well microtitre plates. The effect of chlorhexidine and hydrogen peroxide treatments on the viability of sessile B. cenocepacia cells was clearly reduced compared to the effects on planktonic cells. Treatments with low concentrations of sodium hypochlorite (0.05%, 5 min) and acetic acid (1.25%, 15 min) also resulted in insufficient reductions in the number of viable sessile cells. There was no relation between the ability of the disinfectants to remove biofilm biomass and their potential to kill biofilm cells. In conclusion, our study indicates that testing of the efficacy of disinfectants should be performed on both planktonic and sessile cells, with particular attention to their effects on cellular viability.
在本研究中，我们使用浮游态和固着态洋葱伯克霍尔德菌细胞，评估了推荐用于囊性纤维化患者呼吸设备及其他物品消毒的各种程序的效果。采用改良的欧洲悬浮试验来确定消毒程序对浮游态细胞的影响。使用两种基于刃天青的活力测定法以及对在96孔微量滴定板中生长和处理的生物膜进行结晶紫染色，来评估这些处理杀死固着态细胞及去除生物膜生物量的能力。与对浮游态细胞的影响相比，洗必泰和过氧化氢处理对固着态洋葱伯克霍尔德菌细胞活力的影响明显降低。低浓度次氯酸钠（0.05%，5分钟）和乙酸（1.25%，15分钟）处理也导致固着态活细胞数量减少不足。消毒剂去除生物膜生物量的能力与其杀死生物膜细胞的潜力之间没有关联。总之，我们的研究表明，消毒剂效果测试应同时在浮游态和固着态细胞上进行，尤其要关注其对细胞活力的影响。
