Ammons Mary Cloud B, Ward Loren S, Fisher Steve T, Wolcott Randall D, James Garth A
Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA.
Int J Antimicrob Agents. 2009 Mar;33(3):230-6. doi: 10.1016/j.ijantimicag.2008.08.013. Epub 2008 Nov 1.
The medical impact of bacterial biofilms has increased with the recognition of biofilms as a major contributor to chronic wounds such as diabetic foot ulcers, venous leg ulcers and pressure ulcers. Traditional methods of treatment have proven ineffective, therefore this article presents in vitro evidence to support the use of novel antimicrobials in the treatment of Pseudomonas aeruginosa biofilm. An in vitro biofilm model with a clinical isolate of P. aeruginosa was subjected to treatment with either lactoferrin or xylitol alone or in combination. Combined lactoferrin and xylitol treatment disrupted the structure of the P. aeruginosa biofilm and resulted in a >2log reduction in viability. In situ analysis indicated that while xylitol treatment appeared to disrupt the biofilm structure, lactoferrin treatment resulted in a greater than two-fold increase in the number of permeabilised bacterial cells. The findings presented here indicated that combined treatment with lactoferrin and xylitol significantly decreases the viability of established P. aeruginosa biofilms in vitro and that the antimicrobial mechanism of this treatment includes both biofilm structural disruption and permeablisation of bacterial membranes.
随着生物膜被确认为导致慢性伤口（如糖尿病足溃疡、下肢静脉溃疡和压疮）的主要因素，细菌生物膜对医学的影响日益增加。传统治疗方法已被证明无效，因此本文提供了体外证据，以支持使用新型抗菌剂治疗铜绿假单胞菌生物膜。用临床分离的铜绿假单胞菌建立体外生物膜模型，分别用乳铁蛋白或木糖醇单独处理或联合处理。乳铁蛋白和木糖醇联合处理破坏了铜绿假单胞菌生物膜的结构，并使存活率降低了>2个对数。原位分析表明，虽然木糖醇处理似乎破坏了生物膜结构，但乳铁蛋白处理使细菌细胞通透性增加的数量增加了两倍以上。此处的研究结果表明，乳铁蛋白和木糖醇联合处理可显著降低体外已形成的铜绿假单胞菌生物膜的存活率，且该处理的抗菌机制包括生物膜结构破坏和细菌细胞膜通透性增加。
