果蝇成肌细胞融合的实时成像。
Live imaging of Drosophila myoblast fusion.
作者信息
Richardson Brian E, Beckett Karen, Baylies Mary K
机构信息
Program in Developmental Biology, Memorial Sloan Kettering Institute and Weill Graduate School at Cornell Medical School, New York, NY, USA.
出版信息
Methods Mol Biol. 2008;475:263-74. doi: 10.1007/978-1-59745-250-2_15.
Abstract
Myoblast fusion requires a number of cellular behaviors, including cell migration, recognition, and adhesion, as well as a series of subcellular behaviors, such as cytoskeletal rearrangements, vesicle trafficking, and membrane dynamics, leading to two cells becoming one. With the discovery of fluorescent proteins that can be introduced and studied within living cells, the possibility of monitoring these complex processes within the living embryo is now a reality. Live imaging, unlike imaging techniques for fixed embryos, allows the opportunity to visualize and measure the dynamics of these processes in vivo. This chapter describes the development and use of live imaging techniques to study myoblast fusion in Drosophila.
摘要
成肌细胞融合需要多种细胞行为，包括细胞迁移、识别和黏附，以及一系列亚细胞行为，如细胞骨架重排、囊泡运输和膜动力学，最终导致两个细胞合二为一。随着可导入活细胞并在其中进行研究的荧光蛋白的发现，在活胚胎中监测这些复杂过程已成为现实。与固定胚胎的成像技术不同，实时成像使人们有机会在体内可视化并测量这些过程的动态变化。本章介绍了用于研究果蝇成肌细胞融合的实时成像技术的开发与应用。
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