从具有早期胚胎致死性的小鼠胚胎中建立细胞系。
Establishment of cell lines from mouse embryos with early embryonic lethality.
作者信息
Howerton Kyle, Schlaepfer David D, Ilic Dusko
机构信息
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA.
出版信息
Cell Commun Adhes. 2008 Nov;15(4):379-83. doi: 10.1080/15419060802440054.
Abstract
It is often difficult to determine molecular mechanisms leading to early embryonic lethality of genetically modified mice due to lack of cells for further analyses. The authors describe here establishment of mouse embryonic fibroblast (MEF) cell lines from gastrulation stage embryos. In this example, using a combination of in vivo and in vitro techniques, the authors successfully generated MEF cell lines that lack both fibronectin (FN) and focal adhesion kinase (FAK).
摘要
由于缺乏用于进一步分析的细胞，通常很难确定导致基因编辑小鼠早期胚胎致死的分子机制。作者在此描述了从原肠胚形成阶段胚胎建立小鼠胚胎成纤维细胞（MEF）系的方法。在这个例子中，作者使用体内和体外技术相结合的方法，成功地生成了同时缺乏纤连蛋白（FN）和粘着斑激酶（FAK）的MEF细胞系。
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