Wuhrer Manfred, de Boer Arjen R, Deelder André M
Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Department of Parasitology, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Mass Spectrom Rev. 2009 Mar-Apr;28(2):192-206. doi: 10.1002/mas.20195.
Hydrophilic interaction chromatography (HILIC) with mass spectrometry is a versatile technique for structural glycomics. Glycans are retained by hydrogen bonding, ionic interactions, and dipole-dipole interactions. Glycopeptides as well as glycans with various modifications and reducing-end labels can be efficiently separated, which often results in the resolution of isobaric species. Chromatography is usually performed with solvent mixtures of organic modifier (often acetonitrile) and volatile (acidic) buffer which are suitable for online-electrospray ionization-mass spectrometry. When performed at the nano-scale, this results in a detection limit for oligosaccharides of approximately 1 femtomol. Alternatively, glycans may be analyzed by offline-MALDI-MS(/MS) in both negative-ion mode and positive-ion mode, which allows the registration of informative fragment ion spectra from deprotonated species and sodium adducts, respectively. (c) 2009 Wiley Periodicals, Inc., Mass Spec Rev 28:192-206, 2009.
亲水作用色谱法（HILIC）结合质谱是一种用于结构糖组学研究的通用技术。聚糖通过氢键、离子相互作用和偶极 - 偶极相互作用得以保留。糖肽以及具有各种修饰和还原端标记的聚糖能够被有效分离，这常常使得等压物质得以分离。色谱分析通常使用有机改性剂（通常为乙腈）和挥发性（酸性）缓冲液的混合溶剂进行，这些溶剂适用于在线电喷雾电离质谱分析。当在纳升规模下进行时，这使得寡糖的检测限约为1飞摩尔。另外，聚糖也可以通过离线基质辅助激光解吸电离质谱（/串联质谱）在负离子模式和正离子模式下进行分析，这分别允许记录来自去质子化物种和钠加合物的信息丰富的碎片离子谱。(c) 2009威利期刊公司，《质谱评论》28:192 - 206，2009年。
