Kumar Anil, Sehgal Neha, Kumar Puneet, Padi S S V, Naidu P S
Pharmacology Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160014, India.
Phytother Res. 2008 Dec;22(12):1563-9. doi: 10.1002/ptr.2454.
Intracerebroventricular (i.c.v.) administration of colchicine, a microtubule-disrupting agent, causes cognitive dysfunction and oxidative stress. The present study was designed to investigate the protective effects of quercetin against colchicine-induced memory impairment and oxidative damage in rats. An i.c.v. cannula was implanted in the lateral ventricle of male Wistar rats. Colchicine was administered at dose of 15 microg/rat. Morris water maze and plus-maze performance tests were used to assess memory tasks. Various biochemical parameters such as lipid peroxidation, reduced glutathione, nitrite level, acetylcholinesterase and proteins were also assessed. Central administration of colchicine (15 microg/rat) showed poor retention of memory. Chronic treatment with quercetin (20 and 40 mg/kg, p.o.) twice daily for a period of 25 days beginning 4 days prior to colchicine injection significantly improved the colchicine-induced cognitive impairment. Biochemical analysis revealed that i.c.v. colchicine injection significantly increased lipid peroxidation, nitrite and depleted reduced glutathione activity in the brains of rats. Chronic administration of quercetin significantly attenuated elevated lipid peroxidation and restored the depleted reduced glutathione, acetylcholinesterase activity and nitrite activity. The results of the present study clearly indicated that quercetin has a neuroprotective effect against colchicine-induced cognitive dysfunctions and oxidative damage. This article was published online on 3 November 2008. An error was subsequently identified. This notice is included in the online and print version to indicate that both have been corrected.
向脑室内（i.c.v.）注射秋水仙碱（一种微管破坏剂）会导致认知功能障碍和氧化应激。本研究旨在探讨槲皮素对秋水仙碱诱导的大鼠记忆损伤和氧化损伤的保护作用。将i.c.v.套管植入雄性Wistar大鼠的侧脑室。以15微克/只大鼠的剂量注射秋水仙碱。采用莫里斯水迷宫和十字迷宫行为测试来评估记忆任务。还评估了各种生化参数，如脂质过氧化、还原型谷胱甘肽、亚硝酸盐水平、乙酰胆碱酯酶和蛋白质。脑室内注射秋水仙碱（15微克/只大鼠）显示记忆保持不佳。在注射秋水仙碱前4天开始，每天两次口服槲皮素（20和40毫克/千克），持续25天，可显著改善秋水仙碱诱导的认知障碍。生化分析表明，脑室内注射秋水仙碱可显著增加大鼠脑内脂质过氧化、亚硝酸盐水平，并降低还原型谷胱甘肽活性。长期给予槲皮素可显著减轻脂质过氧化升高，并恢复降低的还原型谷胱甘肽、乙酰胆碱酯酶活性和亚硝酸盐活性。本研究结果清楚地表明，槲皮素对秋水仙碱诱导的认知功能障碍和氧化损伤具有神经保护作用。本文于2008年11月3日在线发表。随后发现一处错误。此通知包含在在线版和印刷版中，以表明两者均已更正。
