用于慢性克氏锥虫感染的聚合酶链反应在血凝块中比在血沉棕黄层或全血标本中具有更高的灵敏度。
Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens.
作者信息
Fitzwater Sean, Calderon Maritza, Lafuente Carlos, Galdos-Cardenas Gerson, Ferrufino Lisbeth, Verastegui Manuela, Gilman Robert H, Bern Caryn
机构信息
Department of International Health, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA.
出版信息
Am J Trop Med Hyg. 2008 Nov;79(5):768-70.
Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.
克氏锥虫聚合酶链反应（PCR）应用广泛，但灵敏度差异很大。我们比较了使用针对玻利维亚女性全血、血沉棕黄层和血凝块中线粒体小环DNA的121/122引物进行的PCR。血凝块中的灵敏度（60.1%）显著高于血沉棕黄层（46.5%）或全血（40%）。使用血凝块可以简化样本采集，同时提高灵敏度。
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