Qi Y M, Lei T, Zhou L, Chen X D, Long Q Q, Long H, Jin D, Gan L, Yang Z Q
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
Mol Biol Rep. 2009 Sep;36(7):1881-8. doi: 10.1007/s11033-008-9394-1. Epub 2008 Nov 4.
CREB3L4 (cAMP response element binding protein3 like 4) belongs to mammalian CREB/ATF subfamily transcription factors featuring a unique putative transmembrane domain lined to the bZIP region in their C-terminals. We report here the cloning of porcine CREB3L4 and its alternative splicing variant. The open reading frame of normal CREB3L4 covers 1,188 bp and encodes a 395-amino acid polypeptide, whereas its variant encodes a C-terminal truncated protein comprising only 271 amino acids. The genomic sequence of porcine CREB3L4 spans approximately 6 kb consisted of ten exons and nine introns, and maps closely to Jumping translocation breakpoint gene in a head-to-head manner on the q arm of Sus Scrofa chromosome 4. Tissue expression analysis by RT-PCR indicates that normal porcine CREB3L4 is ubiquitously expressed with relatively high abundance in stomach, liver, cerebellum, and is a predominant form in various tissues, whereas the splicing variant is expressed at extremely low level in all examined tissues. Our study will lay the groundwork for the further investigation on the physiological function of CREB3L4 in pig models.
CREB3L4（环磷酸腺苷反应元件结合蛋白3样蛋白4）属于哺乳动物CREB/ATF亚家族转录因子，其C末端具有一个与bZIP区域相连的独特假定跨膜结构域。我们在此报告猪CREB3L4及其可变剪接变体的克隆。正常CREB3L4的开放阅读框覆盖1188 bp，编码一个395个氨基酸的多肽，而其变体编码一个仅包含271个氨基酸的C末端截短蛋白。猪CREB3L4的基因组序列跨度约6 kb，由10个外显子和9个内含子组成，并以头对头的方式紧密定位在猪4号染色体q臂上的跳跃易位断点基因附近。通过逆转录聚合酶链反应（RT-PCR）进行的组织表达分析表明，正常猪CREB3L4在胃、肝脏、小脑等组织中广泛表达且丰度相对较高，是各组织中的主要形式，而可变剪接变体在所有检测组织中的表达水平极低。我们的研究将为进一步研究猪模型中CREB3L4的生理功能奠定基础。
