番鸭性腺分化与发育相关基因的转录组分析。
Transcriptome analysis of genes related to gonad differentiation and development in Muscovy ducks.
机构信息
College of Animal Sciences, Fujian Agricultural and Forestry University, Fuzhou, 350002, China.
出版信息
BMC Genomics. 2020 Jun 26;21(1):438. doi: 10.1186/s12864-020-06852-z.
BACKGROUND
Sex-related genes play a crucial role in gonadal differentiation into testes or ovaries. However, the genetic control of gonadal differentiation in Muscovy ducks remains unknown. Therefore, the objective of our study was to screen new candidate genes associated with ovarian and testicular development.
RESULTS
In this study, 24 males before gonadal differentiation (MB), 24 females before gonadal differentiation (FB), 24 males after gonadal differentiation (MA) and 24 females after gonadal differentiation (FA) were selected from Putian Muscovy ducks, forming 4 groups. RNA-Seq revealed 101.76 Gb of clean reads and 2800 differentially expressed genes (DEGs), including 46 in MB vs FB, 609 in MA vs FA, 1027 in FA vs FB, and 1118 in MA vs MB. A total of 146 signalling pathways were enriched by KEGG analysis, among which 20, 108, 108 and 116 signalling pathways were obtained in MB vs FB, MA vs MB, MA vs FA and FA vs FB, respectively. In further GO and KEGG analyses, a total of 21 candidate genes related to gonad differentiation and development in Muscovy ducks were screened. Among these, 9 genes were involved in the differentiation and development of the testes, and 12 genes were involved in the differentiation and development of the ovaries. In addition, RNA-Seq data revealed 2744 novel genes.
CONCLUSIONS
RNA-Seq data revealed 21 genes related to gonadal differentiation and development in Muscovy ducks. We further identified 12 genes, namely, WNT5B, HTRA3, RSPO3, BMP3, HNRNPK, NIPBL, CREB3L4, DKK3, UBE2R2, UBPL3KCMF1, ANXA2, and OSR1, involved in the differentiation and development of ovaries. Moreover, 9 genes, namely, TTN, ATP5A1, DMRT1, DMRT3, AMH, MAP3K1, PIK3R1, AGT and ADAMTSL1, were related to the differentiation and development of testes. Moreover, after gonadal differentiation, DMRT3, AMH, PIK3R1, ADAMTSL1, AGT and TTN were specifically highly expressed in males. WNT5B, ANXA2 and OSR1 were specifically highly expressed in females. These results provide valuable information for studies on the sex control of Muscovy ducks and reveal novel candidate genes for the differentiation and development of testes and ovaries.
背景
与性相关的基因在性腺分化为睾丸或卵巢中起着至关重要的作用。然而，麝香鸭性腺分化的遗传控制尚不清楚。因此，本研究的目的是筛选与卵巢和睾丸发育相关的新候选基因。
结果
本研究从莆田麻鸭中选择了 24 只雄性未分化前（MB）、24 只雌性未分化前（FB）、24 只雄性分化后（MA）和 24 只雌性分化后（FA），共 4 组。RNA-Seq 共获得了 101.76Gb 的清洁读数和 2800 个差异表达基因（DEGs），包括 MB 与 FB 之间的 46 个、MA 与 FA 之间的 609 个、FA 与 FB 之间的 1027 个和 MA 与 MB 之间的 1118 个。KEGG 分析共富集了 146 个信号通路，其中 MB 与 FB 之间、MA 与 MB 之间、MA 与 FA 之间和 FA 与 FB 之间分别获得了 20、108、108 和 116 个信号通路。在进一步的 GO 和 KEGG 分析中，共筛选出 21 个与麝香鸭性腺分化和发育相关的候选基因。其中，9 个基因参与睾丸分化和发育，12 个基因参与卵巢分化和发育。此外，RNA-Seq 数据还揭示了 2744 个新基因。
结论
RNA-Seq 数据揭示了 21 个与麝香鸭性腺分化和发育相关的基因。我们进一步鉴定了 12 个基因，即 WNT5B、HTRA3、RSPO3、BMP3、HNRNPK、NIPBL、CREB3L4、DKK3、UBE2R2、UBPL3、KCMF1、ANXA2 和 OSR1，它们参与了卵巢的分化和发育。此外，9 个基因，即 TTN、ATP5A1、DMRT1、DMRT3、AMH、MAP3K1、PIK3R1、AGT 和 ADAMTSL1，与睾丸的分化和发育有关。此外，在性腺分化后，DMRT3、AMH、PIK3R1、ADAMTSL1、AGT 和 TTN 在雄性中特异性高表达。WNT5B、ANXA2 和 OSR1 在雌性中特异性高表达。这些结果为研究麝香鸭的性别控制提供了有价值的信息，并揭示了睾丸和卵巢分化发育的新候选基因。
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