由脂质包裹的油包水微滴构建不对称细胞大小的脂质囊泡。

Construction of asymmetric cell-sized lipid vesicles from lipid-coated water-in-oil microdroplets.

作者信息

Hamada Tsutomu, Miura Yoko, Komatsu Yuuki, Kishimoto Yuko, Vestergaard Mun'delanji, Takagi Masahiro

出版信息

J Phys Chem B. 2008 Nov 27;112(47):14678-81. doi: 10.1021/jp807784j.

Abstract

We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained water-soluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.

摘要

我们提出了一种简单、快速且稳健的方法，用于通过穿过油水界面转移的油包水（W/O）微滴制备不对称的细胞大小的脂质双层囊泡。阐明了产生细胞大小模型膜的效率与囊泡大小和所含水溶性分子重量的关系。我们使用荧光显微镜证明了其生物学不对称性质以及脂质筏微区结构的形成。