Hanington Patrick C, Hitchen Steven J, Beamish Leigh A, Belosevic Miodrag
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
Fish Shellfish Immunol. 2009 Jan;26(1):1-9. doi: 10.1016/j.fsi.2008.09.020. Epub 2008 Oct 17.
We recently characterized macrophage colony stimulating factor (CSF-1) of fish (the goldfish). Here, we report for the first time that goldfish CSF-1 acts through the CSF-1 receptor by showing loss of CSF-1 function in CSF-1R knockdown monocytes using RNAi, and demonstrate that goldfish CSF-1 administration in vivo increases the amount of circulating monocytes in blood. We also show that conditioned supernatants from goldfish fibroblast cultures induced the proliferation of goldfish monocytes indicating that, like in mammals, teleost fibroblasts are an important producer of CSF-1. The continuous addition of recombinant CSF-1 to primary goldfish macrophage cultures stabilized and extended their longevity and resulted in a long-term culture of functional macrophages capable of mounting a potent nitric oxide response upon activation with goldfish recombinant TNF-alpha.
我们最近对鱼类（金鱼）的巨噬细胞集落刺激因子（CSF-1）进行了特性描述。在此，我们首次报告金鱼CSF-1通过CSF-1受体发挥作用，这是通过RNA干扰显示CSF-1R敲低的单核细胞中CSF-1功能丧失得以证明的，并且证明在体内给予金鱼CSF-1会增加血液中循环单核细胞的数量。我们还表明，金鱼成纤维细胞培养物的条件上清液可诱导金鱼单核细胞增殖，这表明与哺乳动物一样，硬骨鱼成纤维细胞是CSF-1的重要产生者。向原代金鱼巨噬细胞培养物中持续添加重组CSF-1可稳定并延长其寿命，并产生能够在用金鱼重组TNF-α激活后产生强大一氧化氮反应的功能性巨噬细胞的长期培养物。
