School of Fisheries, Zhejiang Ocean University, Zhoushan, 316022, China.
National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, No.106 Nanjing Road, Qingdao, 266071, China.
Fish Physiol Biochem. 2024 Feb;50(1):295-305. doi: 10.1007/s10695-024-01313-w. Epub 2024 Feb 22.
Peroxisome proliferator-activated receptor β (pparβ) is a key gene-regulating lipid metabolism pathway, but its function in turbot remains unclear. In this study, the CDS of pparβ was cloned from kidney for the first time. The CDS sequence length was 1533 bp encoding 510 amino acids. Structural analysis showed that the pparβ protein contained a C4 zinc finger and HOLI domain, suggesting that the pparβ gene of turbot has high homology with the PPAR gene of other species. The high expression patterns of pparβ, acox, and cpt-1 at high temperatures, as shown through qPCR, indicated that high temperatures activated the transcriptional activity of pparβ and increased the activity of the acox and cpt-1 genes. The expression of acox and cpt-1 was significantly inhibited when pparβ was downregulated using RNAi technology and inhibitor treatments, suggesting that pparβ positively regulated acox and cpt-1 expression at high temperatures and, thus, modulates lipid catabolism activity. These results demonstrate that pparβ is involved in the regulation of lipid metabolism at high temperatures and expand a new perspective for studying the regulation of lipid metabolism in stress environments of teleost.
过氧化物酶体增殖物激活受体 β（pparβ）是调控脂质代谢途径的关键基因，但它在大菱鲆中的功能尚不清楚。本研究首次从肾脏克隆得到了 pparβ 的 CDS。CDS 序列长度为 1533bp，编码 510 个氨基酸。结构分析表明，pparβ 蛋白含有一个 C4 锌指和 HOLI 结构域，提示大菱鲆的 pparβ 基因与其他物种的 PPAR 基因具有高度同源性。qPCR 结果显示，pparβ、acox 和 cpt-1 在高温下呈高表达模式，表明高温激活了 pparβ 的转录活性，增加了 acox 和 cpt-1 基因的活性。用 RNAi 技术和抑制剂处理下调 pparβ 后，acox 和 cpt-1 的表达显著受到抑制，表明 pparβ 在高温下正向调节 acox 和 cpt-1 的表达，从而调节脂质分解代谢活性。这些结果表明 pparβ 参与了高温下脂质代谢的调节，为研究硬骨鱼类应激环境下脂质代谢的调节提供了新的视角。
