Hensler T, Köller M, Alouf J E, König W
Lehrstuhl für Med. Mikrobiologie und Immunologie, Ruhr-Universität Bochum, F.R.G.
Biochem Biophys Res Commun. 1991 Sep 16;179(2):872-9. doi: 10.1016/0006-291x(91)91899-n.
We studied the influence of different bacterial toxins (alveolysin; toxic shock syndrome toxin 1, TSST-1 and erythrogenic toxin A, ETA) on the expression of heat shock proteins (hsps) in isolated human polymorphonuclear granulocytes (PMNs). As was shown by Western blotting (anti-hsp72) ETA and TSST-1 were potent inducers of hsps at low toxin concentrations (10 ng/ml). Alveolysin led to the expression of hsps at hemolytic concentrations (1 HU; 700 ng/ml) whereas at subhemolytic concentrations (7 ng/ml) no heat shock response was observed. The induction of heat shock proteins was also accompanied by increased mRNA levels for hsp70 as was determined by PCR-analysis.
