Barazzone C, Kantengwa S, Suter S, Polla B S
Allergy Unit, University Hospital, Geneva, Switzerland.
Inflammation. 1996 Jun;20(3):243-62. doi: 10.1007/BF01488202.
Phagocytosis represents a powerful stress for the phagocytic cells. Phagocytosis of Staphylococcus aureus induces a stress response associated with the synthesis of specific heat shock/stress proteins (HSP). Here we investigated the stress response of human monocyte-macrophages (m phi) to Pseudomonas aeruginosa, a bacterium found, as for S. aureus, in the airways of patients suffering cystic fibrosis. P. aeruginosa activated in m phi the production of both extra- and intracellular O2-; increased Interleukin-1 beta and actin, but failed to induce host HSP. Neither S. aureus' exotoxins nor the scavenging property of P. aeruginosa's alginate, but the lower toxicity of P. aeruginosa and/or differential activation of proteine kinase C (PKC) by the two bacteria, might explain their differences in host HSP induction. While O2- is insufficient to induce HSP synthesis in m phi, hydroxyl radicals, generated in the presence of exogenous iron, is a likely additional signal, along with PKC activation, for HSP induction during bacterial phagocytosis.
吞噬作用对吞噬细胞而言是一种强大的应激。金黄色葡萄球菌的吞噬作用会引发一种与特定热休克/应激蛋白（HSP）合成相关的应激反应。在此，我们研究了人类单核细胞巨噬细胞（m phi）对铜绿假单胞菌的应激反应，铜绿假单胞菌与金黄色葡萄球菌一样，可在囊性纤维化患者的气道中发现。铜绿假单胞菌在m phi中激活了细胞外和细胞内超氧阴离子（O2-）的产生；增加了白细胞介素-1β和肌动蛋白，但未能诱导宿主HSP的产生。金黄色葡萄球菌的外毒素以及铜绿假单胞菌藻酸盐的清除特性均不能解释这一现象，而铜绿假单胞菌较低的毒性和/或两种细菌对蛋白激酶C（PKC）的不同激活作用，可能解释了它们在宿主HSP诱导方面的差异。虽然O2-不足以在m phi中诱导HSP合成，但在外源铁存在的情况下产生的羟基自由基，可能是细菌吞噬过程中与PKC激活一起诱导HSP的另一个信号。
