Köller M, Hensler T, König B, Prévost G, Alouf J, König W
Lehrstuhl für Med. Mikrobiologie und Immunologie, Arbeitsgruppe Infektabwehrmechanismen, Ruhr-Universität Bochum, Germany.
Zentralbl Bakteriol. 1993 Apr;278(2-3):365-76. doi: 10.1016/s0934-8840(11)80853-4.
We studied the influence of a lipid mediator (12-hydroxyeicosatetraenoic acid, 12-HETE), cytokines (IL-6 and TNF-alpha) and different bacterial toxins (alveolysin; exfoliative toxin; toxic shock syndrome toxin 1, TSST-1 and erythrogenic toxin A, ETA) on the expression of heat shock proteins (hsps) in isolated human leucocytes. 12-HETE induces the expression of individual heat shock proteins (65- and 83 kDa) protein in human leukocytes (lymphocytes, monocytes, basophilic granulocytes; LMBs). As was shown by Western blotting (anti-hsp72), IL-6 or TNF-alpha induced hsps preferentially in human LMBs and PMNs, respectively. Among the toxins, ETA and TSST-1 were potent inducers of hsps at low toxin concentrations (10 ng/ml). Alveolysin led to the expression of hsps at hemolytic concentrations (1 HU; 700 ng/ml) whereas at subhemolytic concentrations (7 ng/ml), no heat shock response was observed. The induction of heat shock proteins was also accompanied by increased mRNA levels for hsp70 as determined by PCR analysis. In contrast, exfoliative toxin led to a reduction of the hsp signal in PMNs as determined by Western blotting. Finally, it was demonstrated that PMNs which had been pretreated with TNF-alpha and therefore expressed intracellular hsps were more resistant to cytolytic attack by leukocidin than untreated cells.
我们研究了脂质介质（12-羟基二十碳四烯酸，12-HETE）、细胞因子（IL-6和TNF-α）以及不同细菌毒素（肺泡溶素；剥脱毒素；中毒性休克综合征毒素1，TSST-1和致热外毒素A，ETA）对分离的人白细胞中热休克蛋白（hsps）表达的影响。12-HETE可诱导人白细胞（淋巴细胞、单核细胞、嗜碱性粒细胞；LMBs）中个别热休克蛋白（65 kDa和83 kDa）的表达。如蛋白质免疫印迹法（抗hsp72）所示，IL-6或TNF-α分别优先在人LMBs和中性粒细胞中诱导hsps的表达。在这些毒素中，ETA和TSST-1在低毒素浓度（10 ng/ml）时是hsps的有效诱导剂。肺泡溶素在溶血浓度（1 HU；700 ng/ml）时可导致hsps的表达，而在亚溶血浓度（7 ng/ml）时，未观察到热休克反应。通过PCR分析确定，热休克蛋白的诱导还伴随着hsp70 mRNA水平的升高。相反，如蛋白质免疫印迹法所确定的，剥脱毒素导致中性粒细胞中hsp信号的降低。最后，证明了用TNF-α预处理并因此表达细胞内热休克蛋白的中性粒细胞比未处理的细胞对白细胞杀素的溶细胞攻击更具抗性。
