用于敲除HIV-1的敲低筛选。
Knockdown screens to knockout HIV-1.
作者信息
Goff Stephen P
机构信息
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, HHSC 1310c, New York, NY 10032, USA.
出版信息
Cell. 2008 Oct 31;135(3):417-20. doi: 10.1016/j.cell.2008.10.007.
Abstract
Three recent screens use siRNAs to identify host genes that are critical for HIV-1 replication. These screens have uncovered hundreds of human genes not previously known to be commandeered by the virus during infection. Although some caveats remain, this screening approach opens up a new landscape of viral-host interactions for future exploration.
摘要
最近的三项筛选利用小干扰RNA（siRNA）来识别对HIV-1复制至关重要的宿主基因。这些筛选发现了数百个人类基因，这些基因在感染过程中以前不被认为会被病毒征用。尽管仍有一些注意事项，但这种筛选方法为未来的探索开辟了病毒-宿主相互作用的新领域。
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