Mercier Romain, Petit Marie-Agnès, Schbath Sophie, Robin Stéphane, El Karoui Meriem, Boccard Frédéric, Espéli Olivier
Centre de Génétique Moléculaire du CNRS, 91198 Gif-sur-Yvette, France.
Cell. 2008 Oct 31;135(3):475-85. doi: 10.1016/j.cell.2008.08.031.
The organization of the Escherichia coli chromosome into insulated macrodomains influences the segregation of sister chromatids and the mobility of chromosomal DNA. Here, we report that organization of the Terminus region (Ter) into a macrodomain relies on the presence of a 13 bp motif called matS repeated 23 times in the 800-kb-long domain. matS sites are the main targets in the E. coli chromosome of a newly identified protein designated MatP. MatP accumulates in the cell as a discrete focus that colocalizes with the Ter macrodomain. The effects of MatP inactivation reveal its role as main organizer of the Ter macrodomain: in the absence of MatP, DNA is less compacted, the mobility of markers is increased, and segregation of Ter macrodomain occurs early in the cell cycle. Our results indicate that a specific organizational system is required in the Terminus region for bacterial chromosome management during the cell cycle.
大肠杆菌染色体组织成绝缘的大结构域会影响姐妹染色单体的分离以及染色体DNA的移动性。在此，我们报告，将末端区域（Ter）组织成一个大结构域依赖于一个名为matS的13bp基序的存在，该基序在这个800kb长的结构域中重复23次。matS位点是一种新鉴定的名为MatP的蛋白质在大肠杆菌染色体中的主要靶点。MatP在细胞中以离散的焦点形式积累，该焦点与Ter大结构域共定位。MatP失活的影响揭示了它作为Ter大结构域主要组织者的作用：在没有MatP的情况下，DNA压缩程度降低，标记物的移动性增加，并且Ter大结构域在细胞周期早期发生分离。我们的结果表明，在细胞周期中，末端区域需要一个特定的组织系统来管理细菌染色体。
