Syed Dastager G, Tang Shu-Kun, Cai Man, Zhi Xiao-Yang, Agasar Dayanand, Lee Jae-Chan, Kim Chang-Jin, Jiang Cheng-Lin, Xu Li-Hua, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):570-3. doi: 10.1099/ijs.0.65449-0.
A Gram-positive, moderately halophilic actinomycete strain, designated YIM 90502(T), was isolated from a sample of muddy soil collected from Gulbarga, Karnataka Province, India, and subjected to a polyphasic taxonomical analysis. The isolate grew optimally at 28 degrees C and in the presence of 10 % (w/v) NaCl. The isolate was characterized chemotaxonomically as having meso-diaminopimelic acid in the cell-wall peptidoglycan and galactose and arabinose as whole-cell sugars. The predominant menaquinone was MK-9(H(4)), while MK-8(H(4)) was found in smaller amounts. The phospholipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C(16 : 0) (49.2 %) and C(17 : 1)omega6c (9.1 %). The DNA G+C content of the isolate was 71.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus Saccharomonospora. 16S rRNA gene sequence similarity between strain YIM 90502(T) and the type strains of Saccharomonospora species ranged from 92.42 % (with Saccharomonospora xinjiangensis CCTCC AA 97021(T)) to 97.45 % (with Saccharomonospora azurea KCTC 9693(T)). Levels of DNA-DNA relatedness between strain YIM 90502(T) and S. azurea KCTC 9693(T), Saccharomonospora halophila DSM 44411(T) and Saccharomonospora paurometabolica DSM 44619(T) were 46.0, 41.0 and 42.5 %, respectively. On the basis of phenotypic, phylogenetic and genotypic data, strain YIM 90502(T) was classified in the genus Saccharomonospora as a member of a novel species, for which the name Saccharomonospora saliphila sp. nov. is proposed, with YIM 90502(T) (=KCTC 19234(T) =DSM 45087(T)) as the type strain.
从印度卡纳塔克邦古尔伯加采集的一份泥质土壤样本中分离出一株革兰氏阳性中度嗜盐放线菌菌株，命名为YIM 90502(T)，并对其进行了多相分类分析。该菌株在28℃和10%（w/v）NaCl存在的条件下生长最佳。该菌株经化学分类鉴定，细胞壁肽聚糖中含有内消旋二氨基庚二酸，全细胞糖为半乳糖和阿拉伯糖。主要的甲基萘醌是MK-9(H(4))，同时发现少量的MK-8(H(4))。磷脂为磷脂酰肌醇、磷脂酰甘油、二磷脂酰甘油和磷脂酰乙醇胺。主要脂肪酸为异-C(16 : 0)（49.2%）和C(17 : 1)ω6c（9.1%）。该菌株的DNA G+C含量为71.8 mol%。基于16S rRNA基因序列构建的系统发育树表明，该菌株属于糖单孢菌属所涵盖的进化分支。菌株YIM 90502(T)与糖单孢菌属各模式菌株的16S rRNA基因序列相似性在92.42%（与新疆糖单孢菌CCTCC AA 97021(T)）至97.45%（与天蓝糖单孢菌KCTC 9693(T)）之间。菌株YIM 90502(T)与天蓝糖单孢菌KCTC 9693(T)、嗜盐糖单孢菌DSM 44411(T)和寡代谢糖单孢菌DSM 44619(T)的DNA-DNA杂交率分别为46.0%、41.0%和42.5%。基于表型、系统发育和基因型数据，菌株YIM 90502(T)被归类为糖单孢菌属的一个新物种成员，为此提出新名称嗜盐糖单孢菌（Saccharomonospora saliphila sp. nov.），以YIM 90502(T)（=KCTC 19234(T)=DSM 45087(T)）作为模式菌株。
