Larrosa Mar, Lodovici Maura, Morbidelli Lucia, Dolara Piero
Department of Pharmacology, University of Florence, Florence, Italy.
Free Radic Res. 2008 Oct;42(10):903-10. doi: 10.1080/10715760802510077.
This paper studied the effect on UV-B ocular damage of 10microM hydrocaffeic acid (HCAF) alone and as a mixture (MIX) (5 microM HCAF+5 microM p-coumaric acid). Since ocular UV-B damage is mediated by reactive oxygen species, the aim was to test if HCAF and MIX could reduce oxidation damage in human conjunctival cells (WKD) in vitro and in cornea and sclera of rabbits in vivo. After UVB irradiation (44 J/m(2)) of WKD cells, 8-oxodG levels in DNA were markedly increased and this effect was attenuated by HCAF and MIX. Rabbit eyes were treated by application of HCAF and MIX drops before UV-B exposure (79 J/m(2)). Corneal and scleral DNA oxidation damage, xanthine-oxidase (XO) activity and malondialdehyde levels (MDA) in corneal tissue and prostaglandin E(2) (PGE(2)) in the aqueous humour were reduced by HCAF alone and in combination with p-coumaric acid, showing their potential as a topical treatment against UV-B damage.
本文研究了单独使用10微摩尔的对羟基苯甲酸（HCAF）以及将其与对香豆酸（5微摩尔HCAF + 5微摩尔对香豆酸）混合（MIX）对UV-B所致眼部损伤的影响。由于眼部UV-B损伤是由活性氧介导的，目的是测试HCAF和MIX是否能在体外对人结膜细胞（WKD）以及在体内对兔角膜和巩膜减少氧化损伤。在对WKD细胞进行UVB照射（44 J/m²）后，DNA中的8-氧代脱氧鸟苷水平显著升高，而HCAF和MIX可减弱这种效应。在UV-B暴露（79 J/m²）前，通过滴注HCAF和MIX对兔眼进行处理。单独使用HCAF以及与对香豆酸联合使用时，均可降低角膜组织中的角膜和巩膜DNA氧化损伤、黄嘌呤氧化酶（XO）活性以及丙二醛水平（MDA），并降低房水中的前列腺素E₂（PGE₂）水平，表明它们作为局部治疗UV-B损伤的潜在作用。
