植物酚类物质4-香豆酸可预防大鼠肠道炎症。

Plant phenolic 4-coumaric acid protects against intestinal inflammation in rats.

作者信息

Luceri C, Guglielmi F, Lodovici M, Giannini L, Messerini L, Dolara P

机构信息

Department of Pharmacology, University of Florence, IT-50139 Florence, Italy.

出版信息

Scand J Gastroenterol. 2004 Nov;39(11):1128-33. doi: 10.1080/00365520410007908.

DOI:10.1080/00365520410007908

PMID:15545172

Abstract

BACKGROUND

Many plants contain significant amounts of 4-coumaric acid (4CA), a compound with antioxidant properties in vitro and in vivo. The aim of this study was to assess the effects of 4CA pretreatment on DNA oxidative stress induced by intestinal inflammation in rodents.

METHODS

4CA (50 mg/kg) was administered to rats for 14 days mixed in the diet. Colitis was induced on days 13 and 14 by administering 6% (w/v) dextran sodium sulphate (DSS) in the drinking water.

RESULTS

In the colon mucosa, DSS treatment increased myeloperoxidase activity (P < 0.05), oxidative DNA damage (P < 0.01), and cyclooxygenase-2 (COX-2) expression (P < 0.01) and reduced superoxide dismutase-2 (SOD-2) expression (P < 0.05). It was found that treatment with 4CA prior to DSS-induced inflammation reduced oxidative DNA damage (P < 0.01), COX-2 over-expression (P < 0.01) and restored SOD-2 gene expression to control levels. Similar effects were observed with nimesulide administered p.o. (5 mg/kg, 1 day before and during DSS treatment). PGE levels in plasma and colon mucosa were increased by DSS treatment and this effect was inhibited by pretreatment with 4-CA (P < 0.01).

CONCLUSIONS

Mild acute intestinal inflammation induced by DSS can be inhibited by 4-CA and this action is associated with the suppression of COX-2 expression and activity.

摘要

背景

许多植物含有大量的4-香豆酸（4CA），该化合物在体外和体内均具有抗氧化特性。本研究的目的是评估4CA预处理对啮齿动物肠道炎症诱导的DNA氧化应激的影响。

方法

将4CA（50mg/kg）混入饲料中给予大鼠14天。在第13天和第14天，通过在饮用水中给予6%（w/v）的葡聚糖硫酸钠（DSS）诱导结肠炎。

结果

在结肠黏膜中，DSS处理增加了髓过氧化物酶活性（P<0.05）、氧化性DNA损伤（P<0.01）和环氧合酶-2（COX-2）表达（P<0.01），并降低了超氧化物歧化酶-2（SOD-2）表达（P<0.05）。发现在DSS诱导炎症之前用4CA处理可减少氧化性DNA损伤（P<0.01）、COX-2过表达（P<0.01），并使SOD-2基因表达恢复到对照水平。口服尼美舒利（5mg/kg，在DSS处理前1天及处理期间）也观察到类似效果。DSS处理使血浆和结肠黏膜中的PGE水平升高，而4-CA预处理可抑制这一作用（P<0.01）。