Jung Won-Seok, Chae Young-Seok, Kim Do-Yun, Seo Sang-Wan, Park Hee-Je, Bae Gi-Sang, Kim Tae-Hyeon, Oh Hyo-Jeong, Yun Ki-Jung, Park Rae-Kil, Kim Jong-Suk, Kim Eun-Cheol, Hwang Sung-Yeon, Park Sung-Joo, Song Ho-Joon
Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju, 570-749 Jeonbuk, South Korea.
World J Gastroenterol. 2008 Oct 28;14(40):6188-94. doi: 10.3748/wjg.14.6188.
To investigate the effect of Gardenia jasminoides (GJ) on cerulein-induced acute pancreatitis (AP) in mice.
C57BL/6 mice weighing 18-20 g were divided into three groups. (1) Normal saline-treated group, (2) treatment with GJ at a dose of 0.1 g/kg, (3) treatment with GJ at a dose of 1 g/kg. GJ was administered orally (n = 6 per group) for 1 wk. Three hours later, the mice were given an intraperitoneal injection of cerulein (50 microg/kg), a stable cholecystokinin (CCK) analogue, every hour for a total of 6 h as described previously. The mice were sacrificed at 6 h after completion of cerulein injections. Blood samples were obtained to determine serum amylase, lipase and cytokine levels. The pancreas was rapidly removed for morphologic examination and scoring. A portion of pancreas was stored at -70 degree and prepared for the measurement of tissue myeloperoxidase (MPO) activity, an indicator of neutrophil sequestration, and for reverse-transcriptase PCR (RT-PCR) and real-time PCR measurements.
Treatment with GJ decreased significantly the severity of pancreatitis and pancreatitis-associated lung injury. Treatment with GJ attenuated the severity of AP compared with saline-treated mice, as shown by reduction in pancreatic edema, neutrophil infiltration, serum amylase and lipase levels, serum cytokine levels, and mRNA expression of multiple inflammatory mediators.
These results suggest that GJ attenuated the severity of AP as well as pancreatitis-associated lung injury.
研究栀子对小鼠雨蛙肽诱导的急性胰腺炎（AP）的影响。
将体重18 - 20 g的C57BL/6小鼠分为三组。（1）生理盐水处理组，（2）以0.1 g/kg剂量的栀子处理组，（3）以1 g/kg剂量的栀子处理组。栀子经口给药（每组n = 6），持续1周。3小时后，如前所述，每小时给小鼠腹腔注射雨蛙肽（50微克/千克），一种稳定的胆囊收缩素（CCK）类似物，共注射6小时。在完成雨蛙肽注射6小时后处死小鼠。采集血样以测定血清淀粉酶、脂肪酶和细胞因子水平。迅速取出胰腺进行形态学检查和评分。将一部分胰腺保存在-70℃，用于测定组织髓过氧化物酶（MPO）活性（中性粒细胞隔离的指标）以及进行逆转录聚合酶链反应（RT-PCR）和实时聚合酶链反应测量。
栀子处理显著降低了胰腺炎的严重程度以及与胰腺炎相关的肺损伤。与生理盐水处理的小鼠相比，栀子处理减轻了AP的严重程度，表现为胰腺水肿减轻、中性粒细胞浸润减少、血清淀粉酶和脂肪酶水平降低、血清细胞因子水平降低以及多种炎症介质的mRNA表达降低。
这些结果表明栀子减轻了AP的严重程度以及与胰腺炎相关的肺损伤。
