Hanbang Body-fluid Research Center, Wonkwang University, Iksan, 540-749 Jeonbuk, South Korea.
Life Sci. 2012 Oct 29;91(17-18):866-71. doi: 10.1016/j.lfs.2012.08.035. Epub 2012 Sep 12.
Acute pancreatitis (AP) is a complicated inflammatory disease that has an unknown underlying pathogenesis. Because alpha-pinene can modulate inflammation, we examined whether alpha-pinene plays a role in AP.
Alpha-pinene was administered intraperitoneally 1h prior to the first injection of cerulein. Once AP developed, cerulein, a stable cholecystokinin analog, was injected hourly over a 6-h period. Blood samples were taken 6h later to determine serum amylase and lipase levels. The pancreas and lungs were rapidly removed for morphological examination, myeloperoxidase assay, and real-time reverse transcription polymerase chain reaction. We also isolated the pancreatic acinar cells using a collagenase solution. Cell viability, and cytokine productions were measured in pancreatic acini.
Intraperitoneal administration of alpha-pinene reduced the pancreatic weight (PW) to body weight (BW) ratio and the serum levels of amylase and lipase. Alpha-pinene treatment also reduced histological damage and myeloperoxidase activity in the pancreas and lungs. Furthermore, alpha-pinene pretreatment reduced the production of pancreatic tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 during cerulein-induced AP. In vitro, alpha-pinene inhibited cerulein-induced cell death and cytokine production in isolated cerulein-treated pancreatic acinar cells.
These findings suggest that alpha-pinene has an anti-inflammatory effect during cerulein-induced AP.
急性胰腺炎（AP）是一种复杂的炎症性疾病，其发病机制尚不清楚。由于α-蒎烯可以调节炎症，我们研究了α-蒎烯是否在 AP 中发挥作用。
α-蒎烯在第一次注射亮抑蛋白酶原前 1 小时通过腹腔内给药。一旦发生 AP，亮抑蛋白酶原，一种稳定的胆囊收缩素类似物，每小时注射一次，持续 6 小时。6 小时后采集血样以确定血清淀粉酶和脂肪酶水平。迅速取出胰腺和肺进行形态学检查、髓过氧化物酶测定和实时逆转录聚合酶链反应。我们还使用胶原酶溶液分离胰腺腺泡细胞。测量胰腺腺泡细胞的细胞活力和细胞因子产生。
腹腔内给予α-蒎烯可降低胰腺重量（PW）与体重（BW）的比值以及血清淀粉酶和脂肪酶水平。α-蒎烯治疗还减轻了胰腺和肺中的组织损伤和髓过氧化物酶活性。此外，α-蒎烯预处理可减少亮抑蛋白酶原诱导的 AP 期间胰腺肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和 IL-6的产生。在体外，α-蒎烯抑制亮抑蛋白酶原诱导的分离的亮抑蛋白酶原处理的胰腺腺泡细胞中的细胞死亡和细胞因子产生。
这些发现表明，α-蒎烯在亮抑蛋白酶原诱导的 AP 中具有抗炎作用。
