Nakanishi Hiroaki, Kido Akira, Ohmori Takeshi, Takada Aya, Hara Masaaki, Adachi Noboru, Saito Kazuyuki
Forensic Science Laboratory of Yamanashi Prefectural Police H.Q., 312-4 Kubonakajima, Isawa, Fuefuki, Yamanashi 406-0036, Japan.
Forensic Sci Int. 2009 Jan 10;183(1-3):20-3. doi: 10.1016/j.forsciint.2008.10.003. Epub 2008 Nov 4.
We have used DNA amplification methods to detect common oral bacterial strains to test for the presence of saliva in forensic samples. Streptococcus salivarius and Streptococcus mutans were detected in various forms of saliva samples, whereas these streptococci were not detected in semen, urine, vaginal fluid, or on skin surfaces. Therefore, we demonstrated that these streptococci are promising new marker for the forensic identification of saliva. Our data indicated that S. salivarius is more reliable than S. mutans as an indicator of saliva presence, because the detection rates for S. salivarius and S. mutans by this method were 100% and 90%, respectively. Furthermore, S. salivarius was detected in all saliva stain samples, whereas S. mutans was only identified in 60% of the stains. Finally, using this method we were able to successfully detect S. salivarius and S. mutans in mock forensic samples. We therefore suggested that this method is useful for the identification of saliva in forensic science.
我们已使用DNA扩增方法来检测常见的口腔细菌菌株，以测试法医样本中是否存在唾液。在各种形式的唾液样本中检测到了唾液链球菌和变形链球菌，而在精液、尿液、阴道分泌物或皮肤表面未检测到这些链球菌。因此，我们证明这些链球菌是用于法医鉴定唾液的有前景的新标志物。我们的数据表明，作为唾液存在的指标，唾液链球菌比变形链球菌更可靠，因为通过这种方法检测唾液链球菌和变形链球菌的检出率分别为100%和90%。此外，在所有唾液污渍样本中均检测到了唾液链球菌，而变形链球菌仅在60%的污渍中被鉴定出来。最后，使用这种方法我们能够在模拟法医样本中成功检测到唾液链球菌和变形链球菌。因此，我们建议这种方法对法医学中唾液的鉴定有用。
