Plhalova Lucie, Stepanova Stanislava, Praskova Eva, Chromcova Lucie, Zelnickova Lenka, Divisova Lenka, Skoric Misa, Pistekova Vladimira, Bedanova Iveta, Svobodova Zdenka
Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic.
ScientificWorldJournal. 2012;2012:728189. doi: 10.1100/2012/728189. Epub 2012 Jun 18.
The aim of this study was to assess the impact of metribuzin in surface waters on fish under experimental conditions. The effects of subchronic exposure to metribuzin on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) and on activity of some biochemical markers (CYP450, EROD) in Danio rerio were investigated during a 28-day toxicity test. Juvenile growth tests were performed on D. rerio according to OECD method number 215. Fish at an initial age of 30 days were exposed to a range of metribuzin concentrations (1.5, 5, 16, 33, and 53 mg L⁻¹). Exposure to metribuzin at 53 mg L⁻¹ was associated with increased mortality. Negative effects with regard to total body weight, length, and the inhibition of specific growth rate were induced at concentrations of 33 and 53 mg L⁻¹. Histopathological examination revealed pathological lesions in the liver in pesticide-exposed fish only at the highest concentration of 53 mg L⁻¹ of metribuzin. Based on the results of growth rate, biochemical markers (CYP450, EROD), and histopathological examination, the lowest observed effect concentration (LOEC) value was 33 mg L⁻¹ and the no observed effect concentration (NOEC) value was 16 mg L⁻¹.
本研究的目的是在实验条件下评估嗪草酮在地表水中对鱼类的影响。在一项为期28天的毒性试验中，研究了斑马鱼亚慢性暴露于嗪草酮对其生长、选定器官（鳃、肾脏、肝脏）组织病理学变化的发展以及一些生化标志物（CYP450、EROD）活性的影响。根据经合组织第215号方法对斑马鱼进行幼鱼生长试验。初始年龄为30天的鱼暴露于一系列嗪草酮浓度（1.5、5、16、33和53 mg L⁻¹）下。暴露于53 mg L⁻¹的嗪草酮会导致死亡率增加。在33和53 mg L⁻¹的浓度下，对鱼的总体重、体长以及特定生长率的抑制产生了负面影响。组织病理学检查显示，仅在嗪草酮浓度最高为53 mg L⁻¹时，暴露于农药的鱼的肝脏出现了病理损伤。根据生长率、生化标志物（CYP450、EROD）和组织病理学检查结果，最低观察到效应浓度（LOEC）值为33 mg L⁻¹，未观察到效应浓度（NOEC）值为16 mg L⁻¹。
