Bellocco Ersilia, Barreca Davide, Laganà Giuseppina, Leuzzi Ugo, Tellone Ester, Ficarra Silvana, Kotyk Arnost, Galtieri Antonio
Department of Organic and Biological Chemistry, University of Messina, Messina, Italy.
Mol Cell Biochem. 2009 Jan;321(1-2):165-71. doi: 10.1007/s11010-008-9930-2. Epub 2008 Nov 6.
The anti-proliferative activity of hesperetin, hesperidin, neohesperidin and rutin was evaluated on human hepatoma cell lines (Hep G2) and correlated to their antioxidant activity. The results obtained showed strong anti-proliferative effects of hesperidin and neohesperidin, considerably higher than the other two additives. Hesperetin induced caspase-3 activation, release of LDH and endogenous accumulation of putrescine. Cell cycle distribution seems to indicate that the inhibitory effects of polyphenols on cell growth could be due to G0/G1 block, and activation of apoptotic pathway in the presence of hesperetin. Our results underline also that the glycone forms show reduced scavenging activity against DPPH, but present a remarkable inhibition of cell proliferation and low cytotoxicity.
在人肝癌细胞系（Hep G2）上评估了橙皮素、橙皮苷、新橙皮苷和芦丁的抗增殖活性，并将其与它们的抗氧化活性相关联。所得结果表明，橙皮苷和新橙皮苷具有很强的抗增殖作用，大大高于其他两种添加剂。橙皮素诱导半胱天冬酶-3活化、乳酸脱氢酶释放和腐胺的内源性积累。细胞周期分布似乎表明，多酚对细胞生长的抑制作用可能是由于G0/G1期阻滞，以及在存在橙皮素的情况下凋亡途径的激活。我们的结果还强调，糖苷形式对DPPH的清除活性降低，但具有显著的细胞增殖抑制作用和低细胞毒性。
