Zhang Chun-Lei, Wang Yan-Hong, Chen Hong, Lan Xian-Yong, Lei Chu-Zhao, Fang Xing-Tang
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi, People's Republic of China.
Mol Biol Rep. 2009 Sep;36(7):1839-43. doi: 10.1007/s11033-008-9388-z. Epub 2008 Nov 6.
Melanocortin-4 receptor (MC4R) is one of five G-protein-coupled receptors binding melanocortins that is implicated in the control of feeding behavior and energy homeostasis. Six cattle populations (n = 594), including four Chinese indigenous breeds, Chinese Holstein, and a meat type breed (Angus), were used to detect single nucleotide polymorphisms in 5'-untranslated region of MC4R gene by means of PCR-SSCP and DNA sequencing. Four linked SNPs (g.[-293C>G; -193A>T; -192T>G; -129A>G]) were identified. The g.-293C>G and g.-129A>G could be genotyped with a PCR-RFLP using TaiI in three combined genotypes (AA, AB and BB). The two linked SNPs were associated with body weight and daily gain in Nanyang aged 6 months (P < 0.05), but they had no significant effect on body weight and daily gain in Nanyang aged 24 months (P > 0.05).
黑皮质素-4受体（MC4R）是与黑皮质素结合的五种G蛋白偶联受体之一，与进食行为和能量稳态的控制有关。本研究利用六个牛群体（n = 594），包括四个中国本土品种、中国荷斯坦牛和一个肉用型品种（安格斯牛），通过PCR-SSCP和DNA测序检测MC4R基因5'-非翻译区的单核苷酸多态性。共鉴定出四个连锁的单核苷酸多态性位点（g.[-293C>G; -193A>T; -192T>G; -129A>G]）。g.-293C>G和g.-129A>G位点可通过TaiI酶切的PCR-RFLP方法进行基因分型，存在三种组合基因型（AA、AB和BB）。这两个连锁的单核苷酸多态性位点与6月龄南阳牛的体重和日增重相关（P < 0.05），但对24月龄南阳牛的体重和日增重无显著影响（P > 0.05）。
