Zhang Chun-Lei, Wang Yan-Hong, Chen Hong, Lei Chu-Zhao, Fang Xing-Tang, Wang Ju-Qiang, Ma Gui-Bian, Niu Hui, Xiao Jie
Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China.
Yi Chuan. 2009 Dec;31(12):1221-5. doi: 10.3724/sp.j.1005.2009.01221.
Pro-opiomelanocortin (POMC) plays an important role on animal ingestive behavior and energy homeostasis. Genetic variation of POMC was detected by PCR-SSCP and DNA sequencing techniques in 480 individuals from seven cattle breeds (i.e., Qinchuan, Nanyang, Jiaxian Red cattle, Jinnan, Luxi, Angus, and Chinese Holstein), and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Nanyang cattle. Three SNPs (811845 C>T, 811821 T>C and 811797 A>G, ref. NW_928357) in linkage were detected in P3 locus, which were located at the 3' flanking region of bovine POMC gene. The body weight at 6-month-old and the average daily gain of 0~6-month-old were higher in Nanyang cattle with the genotype BB than with the genotype AA (P<0.05).
阿黑皮素原（POMC）在动物摄食行为和能量平衡中发挥着重要作用。采用PCR-SSCP和DNA测序技术，对秦川牛、南阳牛、郏县红牛、晋南牛、鲁西牛、安格斯牛和中国荷斯坦牛7个牛品种的480个个体进行了POMC基因变异检测，并对南阳牛候选基因的基因型对生长性状的影响进行了关联分析。在P3位点检测到3个连锁的单核苷酸多态性（SNP）（811845 C>T、811821 T>C和811797 A>G，参考序列NW_928357），这些SNP位于牛POMC基因的3'侧翼区。南阳牛中，基因型为BB的个体6月龄体重和0~6月龄平均日增重高于基因型为AA的个体（P<0.05）。
