Yousef I M, Fisher M M
Can J Biochem. 1976 Dec;54(12):1040-6. doi: 10.1139/o76-152.
Liver cell plasma membranes of male rats were isolated and separated into two fractions, one rich in bile canalicular membranes (BCM) and the other comprising the rest of the plasma membrane (PM). Aliquots of BCM, PM, and microsomes were incubated with deoxycholic, chenodeoxycholic, or cholic acid at bile acid - membrane phospholipid mole ratios up to 100, and the phospholipid solubilization from the PM and from microsomes was linear and apparently nonselective, while that from BCM was biphasic and distinctly selective. Phosphatidyl choline and phosphatidyl ethanolamine made up 90% of the phospholipids solubilized from the BCM at a bile acid - membrane phospholipid mole ratio sufficient to solubilize about 50% of the total phospholipids of the BCM. Of particular interest was the observation that the molecular species and fatty acid composition of the phospholipids solubilized from the BCM under these experimental conditions were similar to those of bile obtained from the same animal, and were quite unlike those solubilized at higher bile acid - phospholipids mole ratios. The data are discussed in terms of the mechanism of the biliary secretion of phospholipids.
分离雄性大鼠的肝细胞质膜，并将其分为两个部分，一部分富含胆小管膜（BCM），另一部分包含质膜（PM）的其余部分。将BCM、PM和微粒体的等分试样与脱氧胆酸、鹅去氧胆酸或胆酸以高达100的胆汁酸-膜磷脂摩尔比孵育，从PM和微粒体中溶解的磷脂呈线性且显然无选择性，而从BCM中溶解的磷脂呈双相且具有明显的选择性。在胆汁酸-膜磷脂摩尔比足以溶解约50%的BCM总磷脂的情况下，磷脂酰胆碱和磷脂酰乙醇胺占从BCM中溶解的磷脂的90%。特别有趣的是观察到，在这些实验条件下从BCM中溶解的磷脂的分子种类和脂肪酸组成与从同一动物获得的胆汁相似，与在较高胆汁酸-磷脂摩尔比下溶解的磷脂完全不同。从磷脂的胆汁分泌机制方面对这些数据进行了讨论。
