Boisseau Sylvie, Kunert-Keil Christiane, Lucke Silke, Bouron Alexandre
Institut des Neurosciences, Inserm U837, Grenoble, France.
Histochem Cell Biol. 2009 Mar;131(3):355-63. doi: 10.1007/s00418-008-0532-6. Epub 2008 Nov 7.
The present study was initiated to gain some information about the tissue distribution of transient receptor potential proteins of C-type (TRPC), a family of voltage-independent cation channels, at the beginning of neurogenesis in the telencephalon of embryonic mice. The mRNAs of all known TRPCs (TRPC1-TRPC7) could be found in the cortex at E13. TRPC1, TRPC3 and TRPC5 were the main isoforms, whereas the mRNAs for TRPC2, TRPC4, TRPC6 and TRPC7 were less abundant. The distribution throughout the cortical wall of TRPC1, TRPC3 and TRPC6 was studied by means of immuno-histochemistry. The data collected pointed to a heterogeneous expression of the channels. Three groups were identified. The first one comprises TRPC1, specifically found in the preplate but only in some post-mitotic neurons. It was mainly observed in a subset of cells distinct from the Cajal-Retzius cells. The second group is composed of TRPC3. It was found in non-neuronal cells and also in dividing (5-bromo-2'-deoxyuridine-positive) cells, indicating that TRPC3 is present in precursor cells. The third group contains TRPC6 detected in neuronal and in dividing non-neuronal cells. Double immunostaining experiments showed that TRPC3-positive cells also express TRPC6. Collectively, this report highlights a specific TRPC expression pattern in the immature cortical wall.
本研究旨在获取有关C型瞬时受体电位蛋白（TRPC）在胚胎小鼠端脑神经发生初期的组织分布信息，TRPC是一类非电压依赖性阳离子通道家族。在胚胎第13天（E13）时，在皮质中可发现所有已知TRPC（TRPC1 - TRPC7）的mRNA。TRPC1、TRPC3和TRPC5是主要的亚型，而TRPC2、TRPC4、TRPC6和TRPC7的mRNA含量较少。通过免疫组织化学方法研究了TRPC1、TRPC3和TRPC6在整个皮质壁的分布。收集的数据表明这些通道存在异质性表达。确定了三组。第一组包括TRPC1，特别存在于前板中，但仅在一些有丝分裂后的神经元中。主要在与Cajal - Retzius细胞不同的细胞亚群中观察到。第二组由TRPC3组成。在非神经元细胞以及正在分裂的（5 - 溴 - 2'-脱氧尿苷阳性）细胞中发现，表明TRPC3存在于前体细胞中。第三组包含在神经元和正在分裂的非神经元细胞中检测到的TRPC6。双重免疫染色实验表明，TRPC3阳性细胞也表达TRPC6。总体而言，本报告突出了未成熟皮质壁中特定的TRPC表达模式。
