Shinde Pramod B, Lee Yoon Mi, Dang Hung The, Hong Jongki, Lee Chong-O, Jung Jee H
College of Pharmacy, Pusan National University, Geumjung-ku, Busan 609-735, Republic of Korea.
Bioorg Med Chem Lett. 2008 Dec 15;18(24):6414-8. doi: 10.1016/j.bmcl.2008.10.082. Epub 2008 Oct 22.
Bioassay-guided chemical investigation of the lipophilic extract of a two-sponge association (Jaspis sp. and Poecillastra sp.) led to the isolation of two new bromotyrosine derivatives (1 and 2), along with known derivatives (3-12). Cyclobispsammaplin A (1) is a cyclic derivative of the previously reported bispsammaplin A (13), while psammaplin M (2) is composed of beta-alanine (or aspartic acid) unit. Compounds 3, 4, 6, 10, and 12 are isolated for the first time from a sponge belonging to the subclass Tetractinomorpha. Structure elucidation was performed by a combination of high resolution mass and 2D NMR (principally COSY, HMBC, HSQC, and NOESY) spectroscopy. Compounds 1-4, 6, 10, and 12 were evaluated for cytotoxicity against a small panel of five human solid tumor cell lines and their activity was compared in relevance to their structure.
对一种双海绵组合（扁海绵属物种和蜂巢海绵属物种）的亲脂性提取物进行生物测定导向的化学研究，导致分离出两种新的溴酪氨酸衍生物（1和2）以及已知衍生物（3 - 12）。环双沙马普林A（1）是先前报道的双沙马普林A（13）的环状衍生物，而沙马普林M（2）由β - 丙氨酸（或天冬氨酸）单元组成。化合物3、4、6、10和12首次从属于四放海绵亚纲的一种海绵中分离得到。通过高分辨率质谱和二维核磁共振（主要是COSY、HMBC、HSQC和NOESY）光谱相结合的方法进行结构解析。对化合物1 - 4、6、10和12针对一小部分五种人类实体瘤细胞系进行细胞毒性评估，并根据其结构比较它们的活性。
