Sauerzweig Steven, Munsch Thomas, Lessmann Volkmar, Reymann Klaus G, Braun Holger
Leibniz Institute for Neurobiology (IfN), Projectgroup Neuropharmacology, Brenneckestr. 6, 39118 Magdeburg, Germany.
Exp Cell Res. 2009 Jan 1;315(1):50-66. doi: 10.1016/j.yexcr.2008.10.007. Epub 2008 Oct 21.
The bone marrow represents an easy accessible source of adult stem cells suitable for various cell based therapies. Several studies in recent years suggested the existence of pluripotent stem cells within bone marrow stem cells (BMSC) expressing marker proteins of both embryonic and tissue committed stem cells. These subpopulations were referred to as MAPC, MIAMI and VSEL-cells. Here we describe SD-BMSC (serumdeprivation-induced BMSC) which are induced as a distinct subpopulation after complete serumdeprivation. SD-BMSC are generated from small-sized nestin-positive BMSC (S-BMSC) organized as round-shaped cells in the top layer of BMSC-cultures. The generation of SD-BMSC is caused by a selective proliferation of S-BMSC and accompanied by changes in both morphology and gene expression. SD-BMSC up-regulate not only markers typical for neural stem cells like nestin and GFAP, but also proteins characteristic for embryonic cells like Oct4 and SOX2. We hypothesize, that SD-BMSC like MAPC, MIAMI and VSEL-cells represent derivatives from a single pluripotent stem cell fraction within BMSC exhibiting characteristics of embryonic and tissue committed stem cells. The complete removal of serum might offer a simple way to specifically enrich this fraction of pluripotent embryonic like stem cells in BMSC cultures.
骨髓是适合多种基于细胞的治疗方法的成人干细胞的一个易于获取的来源。近年来的多项研究表明，骨髓干细胞（BMSC）中存在多能干细胞，这些细胞表达胚胎干细胞和组织定向干细胞的标记蛋白。这些亚群被称为MAPC、MIAMI和VSEL细胞。在此，我们描述血清剥夺诱导的BMSC（SD-BMSC），它是在完全血清剥夺后诱导产生的一个独特亚群。SD-BMSC由在BMSC培养物顶层呈圆形排列的小尺寸巢蛋白阳性BMSC（S-BMSC）产生。SD-BMSC的产生是由S-BMSC的选择性增殖引起的，并伴随着形态和基因表达的变化。SD-BMSC不仅上调神经干细胞典型的标记物如巢蛋白和胶质纤维酸性蛋白（GFAP），还上调胚胎细胞特有的蛋白质如八聚体结合转录因子4（Oct4）和SRY（Y染色体性别决定区）-盒2（SOX2）。我们推测，SD-BMSC与MAPC、MIAMI和VSEL细胞一样，代表了BMSC中单个多能干细胞亚群的衍生物，表现出胚胎干细胞和组织定向干细胞的特征。完全去除血清可能提供一种简单的方法，在BMSC培养物中特异性富集这一类似多能胚胎干细胞的亚群。
