Beghetto Elisa, De Paolis Francesca, Montagnani Francesca, Cellesi Carla, Gargano Nicola
Kenton laboratories, Kenton S.r.l., Rome, Italy.
Microbes Infect. 2009 Jan;11(1):66-73. doi: 10.1016/j.micinf.2008.10.004. Epub 2008 Oct 21.
Mycoplasma pneumoniae is the leading cause of atypical pneumonia in children and young adults. Bacterial colonization can occur in both the upper and the lower respiratory tracts and take place both endemically and epidemically worldwide. Characteristically, the infection is chronic in onset and recovery and both humoral and cell-mediated mechanisms are involved in the response to bacterial colonization. To identify bacterial proteins recognized by host antibody responses, a whole-genome M. pneumoniae library was created and displayed on lambda bacteriophage. The challenge of such a library with sera from individuals hospitalized for mycoplasmal pneumonia allowed the identification of a panel of recombinant bacteriophages carrying B-cell epitopes. Among the already known M. pneumoniae B-cell antigens, our results confirmed the immunogenicity of P1 and P30 adhesins. Also, the data presented in this study localized, within their sequences, the immunodominant epitopes recognized by human immunoglobulins. Furthermore, library screening allowed the identification of four novel immunogenic polypeptides, respectively, encoded by fragments of the MPN152, MPN426, MPN456 and MPN-500 open reading frames, highlighting and further confirming the potential of lambda display technology in antigen and epitope discovery.
肺炎支原体是儿童和青年非典型肺炎的主要病因。细菌定植可发生在上、下呼吸道，在全球范围内呈地方性和流行性发生。其特点是感染起病和恢复均较为缓慢，体液免疫和细胞介导机制均参与对细菌定植的反应。为了鉴定宿主抗体反应识别的细菌蛋白，构建了肺炎支原体全基因组文库并展示在λ噬菌体上。用因支原体肺炎住院患者的血清对该文库进行筛选，从而鉴定出一组携带B细胞表位的重组噬菌体。在已知的肺炎支原体B细胞抗原中，我们的结果证实了P1和P30黏附素的免疫原性。此外，本研究提供的数据在其序列中定位了人类免疫球蛋白识别的免疫显性表位。此外，文库筛选还鉴定出分别由MPN152、MPN426、MPN456和MPN - 500开放阅读框片段编码的四种新型免疫原性多肽，突出并进一步证实了λ展示技术在抗原和表位发现方面的潜力。
