Gao Zhan, Chen Fulin, Zhang Junrui, He Lisheng, Cheng Xiaobin, Ma Qin, Mao Tianqiu
Department of Oral &Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Chang Le Xi Road 146#, Xi'an 710032, PR China.
Br J Oral Maxillofac Surg. 2009 Mar;47(2):116-22. doi: 10.1016/j.bjoms.2008.07.199. Epub 2008 Nov 6.
In this study, cell sheets comprising multilayered living bone marrow stromal cells and extracellular matrix were assembled with tubular coral scaffolds for long bone regeneration. Cell sheet with visible mineralized nodules was harvested and wrapped against tubular coral scaffolds with 5mm diameter and 1.5mm wall thickness. New bone formation was investigated by CT scan and histological observation 8 and 12 weeks after implantation of cell sheet/scaffold. The results showed that cortical bone formed within the constructs for both groups. New bone composed 25.75% of the graft in 8 weeks group, compared to that of 40.01% in 12 weeks group. Histological examination showed that new bone formation was in the manner of endochondral osteogenesis, with woven bone matrix subsequently maturing into fully mineralized compact bone. These findings demonstrated that osteogenic cell sheet could vitalize tubulate coral scaffolds to regenerate bone graft with similar shape and structure to native bone.
在本研究中，将包含多层活骨髓基质细胞和细胞外基质的细胞片与管状珊瑚支架组装在一起，用于长骨再生。收获带有可见矿化结节的细胞片，并将其包裹在直径5mm、壁厚1.5mm的管状珊瑚支架上。在植入细胞片/支架8周和12周后，通过CT扫描和组织学观察研究新骨形成情况。结果显示，两组构建物内均形成了皮质骨。8周组新骨占移植物的25.75%，而12周组为40.01%。组织学检查表明，新骨形成是以软骨内成骨的方式进行的，编织骨基质随后成熟为完全矿化的致密骨。这些发现表明，成骨细胞片可以激活管状珊瑚支架，以再生出形状和结构与天然骨相似的骨移植物。
