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采用细胞片技术进行骨工程修复下颌骨缺损。

Bone engineering by cell sheet technology to repair mandibular defects.

作者信息

Shan Xiuli, Hu Deshan

机构信息

Department of Stomatology, The Fifth People's Hospital of Jinan, Jinan, Shandong 250022, P.R. China.

出版信息

Exp Ther Med. 2017 Nov;14(5):5007-5011. doi: 10.3892/etm.2017.5118. Epub 2017 Sep 15.

DOI:10.3892/etm.2017.5118
PMID:29201205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5704306/
Abstract

Effectiveness of bone engineering by cell sheet technology to repair canine mandibular injuries was investigated. Cell sheets were produced from canine stem cells cultured by density gradient centrifugation and osteoblast induction. A scaffold surface of polylactic-co-glycolic acid (PLGA) copolymer was wrapped with cell sheets. Mandibular injuries were created in 12 healthy dogs followed by implantation of PLGA with (experimental group; n, 6) and without (control group; n, 6) cell sheets. The progression of the implants was examined at 3, 9, and 12 weeks postoperative. At postoperative 12 weeks, the graft mostly replaced the new bone in the experimental group, compact similar to normal bone was formed at lingual position and the broken end of the bone was normally healed. The bone mass in the control group was 2.5, which was significantly lower than the 4.5 in the experimental group. The optical density of the new bone in the experimental group was significantly higher than that in the control group. The experimental group had more haversian canal and red bone marrow and contained a larger number of lamellar bone than the control group. Overall, satisfactory bone engineering containing lamellar bone can be established by cell sheet technology, which is an ideal method to repair mandibular injuries.

摘要

研究了通过细胞片技术进行骨工程修复犬下颌骨损伤的有效性。细胞片由通过密度梯度离心培养并经成骨细胞诱导的犬干细胞制成。聚乳酸-乙醇酸共聚物（PLGA）共聚物的支架表面用细胞片包裹。在12只健康犬中制造下颌骨损伤，然后植入带有（实验组；n = 6）和不带有（对照组；n = 6）细胞片的PLGA。在术后3、9和12周检查植入物的进展情况。术后12周时，实验组中移植物大多被新骨替代，舌侧位置形成了与正常骨相似的致密骨，骨断端正常愈合。对照组的骨量为2.5，明显低于实验组的4.5。实验组中新骨的光密度明显高于对照组。与对照组相比，实验组有更多的哈弗斯管和红骨髓，并且含有更多的板层骨。总体而言，通过细胞片技术可以建立含板层骨的令人满意的骨工程，这是修复下颌骨损伤的理想方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/19c2defc9dcf/etm-14-05-5007-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/43051be71177/etm-14-05-5007-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/1c74f9be4724/etm-14-05-5007-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/23baa4ccaae0/etm-14-05-5007-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/3a991e10693e/etm-14-05-5007-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/19c2defc9dcf/etm-14-05-5007-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/43051be71177/etm-14-05-5007-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/1c74f9be4724/etm-14-05-5007-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/23baa4ccaae0/etm-14-05-5007-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/3a991e10693e/etm-14-05-5007-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8705/5704306/19c2defc9dcf/etm-14-05-5007-g04.jpg

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