Gore Mindy, Hutt-Fletcher Lindsey M
Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA.
Virology. 2009 Jan 5;383(1):162-7. doi: 10.1016/j.virol.2008.10.010. Epub 2008 Nov 7.
Epstein-Barr virus has been documented to encode for ten envelope glycoproteins, gB, gH, gL, gM, gN, gp350, gp42, gp78, gp150 and BMRF2. The BDLF2 open reading frame is also predicted to encode a type II membrane protein but, although found in the virion, it has been described as a component of the tegument. We show here that, as predicted, it is the eleventh envelope glycoprotein of the virus. The full length 65 kDa glycoprotein formed a complex with BMRF2 and, as its homologs in other gammaherpesviruses, was dependent on BMRF2, for authentic processing and transport. Two cleavage products of BDLF2 were also identified in cells and in purified virion particles, one corresponding approximately to the aminoterminal half of the protein, that remained associated with the full length form, and one corresponding to the carboxyterminal glycosylated portion of the protein which did not.
爱泼斯坦-巴尔病毒已被证明可编码十种包膜糖蛋白，即gB、gH、gL、gM、gN、gp350、gp42、gp78、gp150和BMRF2。BDLF2开放阅读框也被预测编码一种II型膜蛋白，尽管在病毒粒子中发现了它，但它被描述为病毒被膜的一个组成部分。我们在此表明，正如所预测的那样，它是该病毒的第十一种包膜糖蛋白。全长65 kDa的糖蛋白与BMRF2形成复合物，并且与其他γ疱疹病毒中的同源物一样，其真实的加工和运输依赖于BMRF2。在细胞和纯化的病毒粒子中还鉴定出BDLF2的两种裂解产物，一种大约对应于该蛋白的氨基末端一半，它仍与全长形式相关联，另一种对应于该蛋白的羧基末端糖基化部分，它则不相关联。
